Restriction map for
pFN21AE5905
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 199 GTAGAC GT^[AC][GT]AC
AocI 864 CCTTAGG CC^T[ACGT]AGG
AseI 514 ATTAAT AT^TAAT
AsnI 514 ATTAAT AT^TAAT
Asp700I 696 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 887 GTGCTC G[AT]GC[AT]^C
BanI 478 GGCACC G^G[CT][GA]CC
BbsI 700 GTCTTC GAAGAC(2/6)
BcgI 276 CGATGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 134 TGATCA T^GATCA
BglI 340 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 780 CTCCAG CTGGAG(16/14)
BpuAI 700 GTCTTC GAAGAC(2/6)
BsaBI 129 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 73 CTCCTC GAGGAG(10/8)
BsgI 284 GTGCAG GTGCAG(16/14)
BsiHKAI 887 GTGCTC G[AT]GC[AT]^C
BsmAI 442 GTCTC GTCTC(1/5)
BsmFI 257 GGGAC GGGAC(10/14)
BsmI 675 GCATTC GAATGC(1/-1)
BspMI 406 GCAGGT ACCTGC(4/8)
BsrDI 245 CATTGC GCAATG(2/0)
BstXI 230 CCAAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 864 CCTTAGG CC^T[ACGT]AGG
EarI 380 CTCTTC CTCTTC(1/4)
Eco81I 864 CCTTAGG CC^T[ACGT]AGG
EcoT14I 344 CCATGG C^C[AT][AT]GG
FbaI 134 TGATCA T^GATCA
Ksp632I 380 CTCTTC CTCTTC(1/4)
MaeII 608 ACGT A^CGT
MamI 129 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 344 CCATGG C^CATGG
NdeI 651 CATATG CA^TATG
PflMI 786 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 514 ATTAAT AT^TAAT
PvuII 218 CAGCTG CAG^CTG
SfcI 221 CTGTAG C^T[GA][CT]AG
SfiI 339 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 376 AGGCCT AGG^CCT
StyI 344 CCATGG C^C[AT][AT]GG
Van91I 786 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 782 CCAGCCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 696 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 249 GCGG CCGC(-3/-1)
367 GCGG
AfaI 171 GTAC GT^AC
765 GTAC
AluI 219 AGCT AG^CT
288 AGCT
BmyI 416 GTGCCC G[AGT]GC[ACT]^C
887 GTGCTC
BsaJI 344 CCATGG C^C[ACGT][ACGT]GG
791 CCGTGG
Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C
887 GTGCTC
BsrI 430 ACTGG ACTGG(1/-1)
786 CCAGT
Cac8I 475 GCAGGC GC[ACGT]^[ACGT]GC
598 GCAGGC
DpnII 135 GATC ^GATC
355 GATC
DsaI 344 CCATGG C^C[GA][CT]GG
791 CCGTGG
EcoT22I 654 ATGCAT ATGCA^T
673 ATGCAT
MboI 135 GATC ^GATC
355 GATC
MseI 43 TTAA T^TAA
515 TTAA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
365 CAGCGG
MwoI 340 GCCTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
418 GCCCTCCTTGC
NdeII 135 GATC ^GATC
355 GATC
NlaIV 433 GGTTCC GG[ACGT]^[ACGT]CC
478 GGCACC
NsiI 654 ATGCAT ATGCA^T
673 ATGCAT
PleI 148 GACTC GAGTC(4/5)
488 GACTC
RsaI 171 GTAC GT^AC
765 GTAC
Sau3AI 135 GATC ^GATC
355 GATC
TfiI 834 GATTC G^A[AT]TC
846 GAATC
Tru9I 43 TTAA T^TAA
515 TTAA
Tsp45I 19 GTGAC ^GT[GC]AC
530 GTGAC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
BbvI BsiYI BslI BssKI BstF5I
BstNI DdeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboII MnlI MslI MvaI NlaIII
ScrFI SfaNI TseI TspRI