Restriction map for
pFN21AE6089
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MflI MluI MluNI
MroI MscI MseI MspA1I MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 447 GCGG CCGC(-3/-1)
AocI 838 CCTGAGG CC^T[ACGT]AGG
AvaII 386 GGACC G^G[AT]CC
BanI 934 GGTGCC G^G[CT][GA]CC
BanII 166 GGGCTC G[GA]GC[CT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvI 326 GCTGC GCAGC(8/12)
BciVI 192 GGATAC GTATCC(6/5)
BclI 617 TGATCA T^GATCA
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaAI 195 TACGTA [CT]AC^GT[GA]
BsaBI 100 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstF5I 99 GGATG GGATG(2/0)
BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 838 CCTGAGG CC^T[ACGT]AGG
DraIII 403 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 389 CCATGG C^C[GA][CT]GG
Eco81I 838 CCTGAGG CC^T[ACGT]AGG
EcoRI 70 GAATTC G^AATTC
EcoT14I 389 CCATGG C^C[AT][AT]GG
EcoT22I 40 ATGCAT ATGCA^T
FbaI 617 TGATCA T^GATCA
FokI 99 GGATG GGATG(9/13)
HinfI 303 GAGTC G^A[ACGT]TC
MaeII 196 ACGT A^CGT
MamI 100 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 532 CAATTG C^AATTG
MunI 532 CAATTG C^AATTG
MwoI 96 GCAGGATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 389 CCATGG C^CATGG
NsiI 40 ATGCAT ATGCA^T
PflMI 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 303 GAGTC GAGTC(4/5)
SapI 537 GCTCTTC GCTCTTC(1/4)
SexAI 143 ACCTGGT A^CC[AT]GGT
SfcI 906 CTACAG C^T[GA][CT]AG
SmlI 912 CTTGAG C^T[CT][GA]AG
SnaBI 195 TACGTA TAC^GTA
StyI 389 CCATGG C^C[AT][AT]GG
TseI 326 GCTGC G^C[AT]GC
Van91I 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 70 GAATTC [GA]^AATT[CT]
962 AAATTT
ApoI 70 GAATTC [GA]^AATT[CT]
962 AAATTT
BmyI 166 GGGCTC G[AGT]GC[ACT]^C
935 GTGCCC
BseRI 115 CTCCTC GAGGAG(10/8)
825 CTCCTC
BsiYI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCCAGGTTCGG
BslI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCCAGGTTCGG
BsmFI 385 GGGAC GGGAC(10/14)
271 GTCCC
Bsp1286I 166 GGGCTC G[AGT]GC[ACT]^C
935 GTGCCC
EarI 220 CTCTTC CTCTTC(1/4)
538 CTCTTC
Fnu4HI 326 GCTGC GC^[ACGT]GC
447 GCGGC
ItaI 326 GCTGC GC^[ACGT]GC
447 GCGGC
Ksp632I 220 CTCTTC CTCTTC(1/4)
538 CTCTTC
SspI 289 AATATT AAT^ATT
496 AATATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI Asp700I BsaJI BssKI
BstNI Cfr13I DdeI DpnII EcoRII
HaeIII HphI MaeIII MboI MboII
MnlI MslI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tsp45I Tsp509I TspRI XmnI