Restriction map for
pFN21AE6037
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 696 CCGC CCGC(-3/-1)
AlwNI 701 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 710 AGCGCT AGC^GCT
Asp700I 717 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 427 GGCACC G^G[CT][GA]CC
BanII 908 GAGCTC G[GA]GC[CT]^C
BbsI 838 GTCTTC GAAGAC(2/6)
BfaI 565 CTAG C^TAG
BpuAI 838 GTCTTC GAAGAC(2/6)
BsaI 174 GAGACC GGTCTC(1/5)
BsmFI 945 GGGAC GGGAC(10/14)
BspHI 44 TCATGA T^CATGA
BspMI 430 ACCTGC ACCTGC(4/8)
BsrBI 696 CCGCTC CCGCTC(-3/-3)
BsrDI 651 CATTGC GCAATG(2/0)
BstEII 801 GGTGACC G^GT[ACGT]ACC
BstPI 801 GGTGACC G^GT[ACGT]ACC
BstXI 242 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 711 GCGC GCG^C
DpnII 373 GATC ^GATC
DrdI 195 GACACACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 362 CCATGG C^C[GA][CT]GG
Eco47III 710 AGCGCT AGC^GCT
EcoO65I 801 GGTGACC G^GT[ACGT]ACC
HaeII 710 AGCGCT [GA]GCGC^[CT]
HhaI 711 GCGC GCG^C
HinP1I 711 GCGC G^CGC
HinfI 920 GAGTC G^A[ACGT]TC
MaeI 565 CTAG C^TAG
MboI 373 GATC ^GATC
NcoI 362 CCATGG C^CATGG
NdeII 373 GATC ^GATC
NheI 564 GCTAGC G^CTAGC
PflMI 14 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 920 GAGTC GAGTC(4/5)
RcaI 44 TCATGA T^CATGA
SacI 908 GAGCTC GAGCT^C
SapI 312 GCTCTTC GCTCTTC(1/4)
Sau3AI 373 GATC ^GATC
SfcI 879 CTACAG C^T[GA][CT]AG
StuI 719 AGGCCT AGG^CCT
TaqI 918 TCGA T^CGA
TthHB8I 918 TCGA T^CGA
Van91I 14 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 137 CCATCATTTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 717 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 37 GAATTT [GA]^AATT[CT]
129 GAATTT
AfaI 108 GTAC GT^AC
222 GTAC
ApoI 37 GAATTT [GA]^AATT[CT]
129 GAATTT
AspHI 576 GTGCTC G[AT]GC[AT]^C
908 GAGCTC
AvaII 23 GGACC G^G[AT]CC
159 GGACC
BalI 134 TGGCCA TGG^CCA
383 TGGCCA
BlpI 698 GCTCAGC GC^T[ACGT]AGC
706 GCTGAGC
Bpu1102I 698 GCTCAGC GC^T[ACGT]AGC
706 GCTGAGC
BsgI 688 CTGCAC GTGCAG(16/14)
734 GTGCAG
BsiHKAI 576 GTGCTC G[AT]GC[AT]^C
908 GAGCTC
CelII 698 GCTCAGC GC^T[ACGT]AGC
706 GCTGAGC
EaeI 134 TGGCCA [CT]^GGCC[GA]
383 TGGCCA
MluNI 134 TGGCCA TGG^CCA
383 TGGCCA
MscI 134 TGGCCA TGG^CCA
383 TGGCCA
MspA1I 446 CAGCTG C[AC]G^C[GT]G
701 CAGCTG
NlaIV 23 GGACCC GG[ACGT]^[ACGT]CC
427 GGCACC
PvuII 446 CAGCTG CAG^CTG
701 CAGCTG
RsaI 108 GTAC GT^AC
222 GTAC
Tsp509I 38 AATT ^AATT
130 AATT
TspRI 236 CAGTG CA[GC]TG[ACGT][ACGT]^
683 CACTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AluI BbvI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MaeIII MboII MnlI MslI MvaI
MwoI NlaIII Sau96I ScrFI SfaNI
StyI TseI Tsp45I