Restriction map for
pFN21AE5929
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 535 GCGG CCGC(-3/-1)
AcsI 68 AAATTC [GA]^AATT[CT]
AgeI 359 ACCGGT A^CCGGT
AlwI 204 GGATC GGATC(4/5)
Aor51HI 453 AGCGCT AGC^GCT
ApoI 68 AAATTC [GA]^AATT[CT]
AspHI 417 GTGCTC G[AT]GC[AT]^C
BanI 435 GGTGCC G^G[CT][GA]CC
BbvI 395 GCAGC GCAGC(8/12)
BcnI 57 CCGGG CC^[GC]GG
BfaI 493 CTAG C^TAG
BglII 329 AGATCT A^GATCT
BmyI 417 GTGCTC G[AGT]GC[ACT]^C
BpmI 241 CTGGAG CTGGAG(16/14)
BsaBI 330 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 472 GAGACC GGTCTC(1/5)
BsaWI 359 ACCGGT [AT]^CCGG[AT]
BseRI 73 CTCCTC GAGGAG(10/8)
BsiHKAI 417 GTGCTC G[AT]GC[AT]^C
BsmAI 472 GAGAC GTCTC(1/5)
BsmFI 42 GGGAC GGGAC(10/14)
Bsp1286I 417 GTGCTC G[AGT]GC[ACT]^C
BsrFI 359 ACCGGT [GA]^CCGG[CT]
BstXI 95 CCATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 454 GCGC GCG^C
Cfr10I 359 ACCGGT [GA]^CCGG[CT]
DraI 672 TTTAAA TTT^AAA
DrdI 44 GACTGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 453 AGCGCT AGC^GCT
EcoNI 138 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 395 GCAGC GC^[ACGT]GC
HaeII 453 AGCGCT [GA]GCGC^[CT]
HhaI 454 GCGC GCG^C
HinP1I 454 GCGC G^CGC
HindIII 704 AAGCTT A^AGCTT
HphI 21 GGTGA GGTGA(8/7)
ItaI 395 GCAGC GC^[ACGT]GC
MaeI 493 CTAG C^TAG
MaeII 16 ACGT A^CGT
MamI 330 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 734 CAGCTG C[AC]G^C[GT]G
NciI 57 CCGGG CC^[GC]GG
NdeI 636 CATATG CA^TATG
NspI 164 ACATGC [GA]CATG^[CT]
PflMI 149 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 359 ACCGGT A^CCGGT
PleI 228 GACTC GAGTC(4/5)
PshAI 732 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 734 CAGCTG CAG^CTG
SfaNI 237 GATGC GCATC(5/9)
SfcI 686 CTACAG C^T[GA][CT]AG
TseI 395 GCAGC G^C[AT]GC
Van91I 149 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 174 GTAC GT^AC
363 GTAC
AseI 517 ATTAAT AT^TAAT
897 ATTAAT
AsnI 517 ATTAAT AT^TAAT
897 ATTAAT
BalI 92 TGGCCA TGG^CCA
368 TGGCCA
BsgI 12 CTGCAC GTGCAG(16/14)
292 GTGCAG
BsrI 460 ACTGG ACTGG(1/-1)
50 CCAGT
BstF5I 141 CATCC GGATG(2/0)
336 CATCC
BstYI 204 GGATCT [GA]^GATC[CT]
329 AGATCT
DpnII 205 GATC ^GATC
330 GATC
EaeI 92 TGGCCA [CT]^GGCC[GA]
368 TGGCCA
EcoT14I 123 CCTTGG C^C[AT][AT]GG
314 CCTTGG
FokI 141 CATCC GGATG(9/13)
336 CATCC
HapII 57 CCGG C^CGG
360 CCGG
HpaII 57 CCGG C^CGG
360 CCGG
MboI 205 GATC ^GATC
330 GATC
MflI 204 GGATCT [GA]^GATC[CT]
329 AGATCT
MluNI 92 TGGCCA TGG^CCA
368 TGGCCA
MscI 92 TGGCCA TGG^CCA
368 TGGCCA
MspI 57 CCGG C^CGG
360 CCGG
MwoI 159 GCTCCACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
343 GCTGTGATGGC
NdeII 205 GATC ^GATC
330 GATC
PshBI 517 ATTAAT AT^TAAT
897 ATTAAT
RsaI 174 GTAC GT^AC
363 GTAC
Sau3AI 205 GATC ^GATC
330 GATC
StyI 123 CCTTGG C^C[AT][AT]GG
314 CCTTGG
TfiI 678 GATTC G^A[AT]TC
793 GAATC
XhoII 204 GGATCT [GA]^GATC[CT]
329 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AvaII BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I DdeI
EarI EcoRII HaeIII HinfI Ksp632I
MaeIII MboII MnlI MseI MslI
MvaI NlaIII NlaIV Sau96I ScrFI
Tru9I Tsp45I Tsp509I TspRI