Restriction map for
pFN21AE5925
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 711 GTCTAC GT^[AC][GT]AC
AciI 147 CCGC CCGC(-3/-1)
AflIII 536 ACACGT A^C[GA][CT]GT
AlwI 807 GGATC GGATC(4/5)
AlwNI 53 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 436 GGGCCC GGGCC^C
AseI 679 ATTAAT AT^TAAT
AsnI 679 ATTAAT AT^TAAT
Asp700I 328 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 436 GGGCCC G[GA]GC[CT]^C
BmyI 436 GGGCCC G[AGT]GC[ACT]^C
BsaAI 364 TACGTA [CT]AC^GT[GA]
BsmAI 540 GTCTC GTCTC(1/5)
BsmBI 539 CGTCTC CGTCTC(1/5)
Bsp1286I 436 GGGCCC G[AGT]GC[ACT]^C
BspHI 587 TCATGA T^CATGA
BsrDI 122 GCAATG GCAATG(2/0)
BstXI 611 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 422 GCGC GCG^C
DraI 774 TTTAAA TTT^AAA
DraII 436 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 61 GAAGAG CTCTTC(1/4)
EcoO109I 436 GGGCCCT [GA]G^G[ACGT]CC[CT]
HhaI 422 GCGC GCG^C
HinP1I 422 GCGC G^CGC
HindIII 704 AAGCTT A^AGCTT
HphI 740 TCACC GGTGA(8/7)
Ksp632I 61 GAAGAG CTCTTC(1/4)
PshBI 679 ATTAAT AT^TAAT
PstI 375 CTGCAG CTGCA^G
RcaI 587 TCATGA T^CATGA
SfaNI 643 GCATC GCATC(5/9)
SfcI 375 CTGCAG C^T[GA][CT]AG
SnaBI 364 TACGTA TAC^GTA
TspRI 432 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 328 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 66 GGTCC G^G[AT]CC
153 GGACC
BbsI 917 GAAGAC GAAGAC(2/6)
310 GTCTTC
BbvI 284 GCTGC GCAGC(8/12)
322 GCTGC
BpmI 157 CTCCAG CTGGAG(16/14)
927 CTCCAG
BpuAI 917 GAAGAC GAAGAC(2/6)
310 GTCTTC
BsmFI 45 GGGAC GGGAC(10/14)
624 GTCCC
BsrI 433 ACTGG ACTGG(1/-1)
394 CCAGT
BssKI 150 CCTGG ^CC[ACGT]GG
929 CCAGG
BstNI 150 CCTGG CC^[AT]GG
929 CCAGG
DpnII 358 GATC ^GATC
808 GATC
EcoRII 150 CCTGG ^CC[AT]GG
929 CCAGG
Fnu4HI 284 GCTGC GC^[ACGT]GC
322 GCTGC
ItaI 284 GCTGC GC^[ACGT]GC
322 GCTGC
MboI 358 GATC ^GATC
808 GATC
MvaI 150 CCTGG CC^[AT]GG
929 CCAGG
NdeII 358 GATC ^GATC
808 GATC
NlaIV 436 GGGCCC GG[ACGT]^[ACGT]CC
607 GGTTCC
Sau3AI 358 GATC ^GATC
808 GATC
ScrFI 150 CCTGG CC^[ACGT]GG
929 CCAGG
TseI 284 GCTGC G^C[AT]GC
322 GCTGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BstF5I Cfr13I DdeI FokI
HaeIII HinfI MaeII MaeIII MboII
MnlI MseI MslI NlaIII PleI
Sau96I TfiI Tru9I Tsp45I Tsp509I