Restriction map for
pFN21AE5385
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssKI BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoRV FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MspI MunI MvaI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 873 CTTAAG C^TTAAG
AlwI 261 GGATC GGATC(4/5)
Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 371 TGGCCA TGG^CCA
BbsI 88 GTCTTC GAAGAC(2/6)
BclI 134 TGATCA T^GATCA
BfrI 873 CTTAAG C^TTAAG
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaI 758 GGTCTC GGTCTC(1/5)
BseRI 624 CTCCTC GAGGAG(10/8)
BsgI 293 CTGCAC GTGCAG(16/14)
BsmFI 233 GTCCC GGGAC(10/14)
Bsp1407I 773 TGTACA T^GTACA
BsrGI 773 TGTACA T^GTACA
BstAPI 286 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 339 CATCC GGATG(2/0)
BstXI 236 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 3 GGGCC G^G[ACGT]CC
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoRI 31 GAATTC G^AATTC
FbaI 134 TGATCA T^GATCA
FokI 339 CATCC GGATG(9/13)
HphI 888 GGTGA GGTGA(8/7)
MaeIII 380 GTAAC ^GT[ACGT]AC
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 298 CAGCTG C[AC]G^C[GT]G
NcoI 350 CCATGG C^CATGG
NlaIV 724 GGTTCC GG[ACGT]^[ACGT]CC
PflFI 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 298 CAGCTG CAG^CTG
Sau96I 3 GGGCC G^G[ACGT]CC
SmlI 873 CTTAAG C^T[CT][GA]AG
SspBI 773 TGTACA T^GTACA
TaqI 264 TCGA T^CGA
TfiI 72 GATTC G^A[AT]TC
Tth111I 416 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 264 TCGA T^CGA
XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
57 GCGG
AflIII 653 ACATGT A^C[GA][CT]GT
718 ACATGT
AlwNI 412 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
463 CAGGCTCTG
BsaJI 236 CCAAGG C^C[ACGT][ACGT]GG
350 CCATGG
BsiYI 76 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BslI 76 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BsmAI 408 GTCTC GTCTC(1/5)
759 GTCTC
BspLU11I 653 ACATGT A^CATGT
718 ACATGT
BsrI 150 ACTGG ACTGG(1/-1)
429 ACTGG
Cac8I 243 GCTGGC GC[ACGT]^[ACGT]GC
300 GCTGGC
EarI 766 CTCTTC CTCTTC(1/4)
883 GAAGAG
EcoT14I 236 CCAAGG C^C[AT][AT]GG
350 CCATGG
EcoT22I 284 ATGCAT ATGCA^T
682 ATGCAT
Ksp632I 766 CTCTTC CTCTTC(1/4)
883 GAAGAG
NsiI 284 ATGCAT ATGCA^T
682 ATGCAT
PleI 154 GACTC GAGTC(4/5)
86 GAGTC
PstI 67 CTGCAG CTGCA^G
689 CTGCAG
StyI 236 CCAAGG C^C[AT][AT]GG
350 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsmI DdeI DpnII Fnu4HI HaeIII
HinfI ItaI MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NspI RsaI Sau3AI SfcI Tru9I
TseI Tsp509I TspRI