Restriction map for
pFN21AE5934
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
BanI BbeI BbrPI BcgI BciVI
BcnI BfrI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AcsI 923 AAATTC [GA]^AATT[CT]
AflIII 316 ACATGT A^C[GA][CT]GT
AlwNI 412 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 923 AAATTC [GA]^AATT[CT]
AspI 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 282 CCTAGG C^CTAGG
BamHI 494 GGATCC G^GATCC
BanII 915 GGGCTC G[GA]GC[CT]^C
BbsI 334 GTCTTC GAAGAC(2/6)
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BclI 635 TGATCA T^GATCA
BfaI 283 CTAG C^TAG
BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 508 AGATCT A^GATCT
BlnI 282 CCTAGG C^CTAGG
BmyI 915 GGGCTC G[AGT]GC[ACT]^C
BpuAI 334 GTCTTC GAAGAC(2/6)
BseRI 219 CTCCTC GAGGAG(10/8)
Bsp1286I 915 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 392 TGTACA T^GTACA
BspHI 632 TCATGA T^CATGA
BspLU11I 316 ACATGT A^CATGT
BspMI 653 ACCTGC ACCTGC(4/8)
BsrGI 392 TGTACA T^GTACA
BssKI 600 CCTGG ^CC[ACGT]GG
BstEII 399 GGTGACC G^GT[ACGT]ACC
BstNI 600 CCTGG CC^[AT]GG
BstPI 399 GGTGACC G^GT[ACGT]ACC
DsaI 753 CCATGG C^C[GA][CT]GG
EcoO65I 399 GGTGACC G^GT[ACGT]ACC
EcoRII 600 CCTGG ^CC[AT]GG
FbaI 635 TGATCA T^GATCA
HincII 808 GTTGAC GT[CT]^[GA]AC
HindII 808 GTTGAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
MaeI 283 CTAG C^TAG
MaeII 112 ACGT A^CGT
MvaI 600 CCTGG CC^[AT]GG
NcoI 753 CCATGG C^CATGG
NspI 316 ACATGT [GA]CATG^[CT]
PflFI 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 689 CTGCAG CTGCA^G
RcaI 632 TCATGA T^CATGA
ScrFI 600 CCTGG CC^[ACGT]GG
SfiI 345 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 392 TGTACA T^GTACA
Tth111I 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 177 GTAC GT^AC
393 GTAC
AlwI 495 GATCC GGATC(4/5)
494 GGATC
BbvI 417 GCTGC GCAGC(8/12)
440 GCTGC
BsgI 15 CTGCAC GTGCAG(16/14)
137 CTGCAC
BsmFI 454 GGGAC GGGAC(10/14)
58 GTCCC
BstF5I 639 CATCC GGATG(2/0)
885 GGATG
BstYI 494 GGATCC [GA]^GATC[CT]
508 AGATCT
EarI 79 CTCTTC CTCTTC(1/4)
499 CTCTTC
Fnu4HI 417 GCTGC GC^[ACGT]GC
440 GCTGC
FokI 639 CATCC GGATG(9/13)
885 GGATG
ItaI 417 GCTGC GC^[ACGT]GC
440 GCTGC
Ksp632I 79 CTCTTC CTCTTC(1/4)
499 CTCTTC
MflI 494 GGATCC [GA]^GATC[CT]
508 AGATCT
MseI 257 TTAA T^TAA
521 TTAA
MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
420 GCGTGTGGAGC
RsaI 177 GTAC GT^AC
393 GTAC
Tru9I 257 TTAA T^TAA
521 TTAA
TseI 417 GCTGC G^C[AT]GC
440 GCTGC
XhoII 494 GGATCC [GA]^GATC[CT]
508 AGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BalI BsaJI BsiYI BslI
BsmAI Cac8I DdeI DpnII EaeI
EcoT14I HaeIII HinfI HphI MaeIII
MboI MboII MluNI MnlI MscI
MslI NdeII NlaIII NlaIV PleI
Sau3AI SfcI StyI TfiI Tsp45I
Tsp509I TspRI