Restriction map for
pFN21AE6011
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FokI FseI
FspI HaeIII HapII HgaI HincII
HindII HindIII HpaI HpaII KpnI
KspI MaeII MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 704 GGCGCC G[GA]^CG[CT]C
AfaI 183 GTAC GT^AC
AflIII 902 ACATGT A^C[GA][CT]GT
AluI 422 AGCT AG^CT
AlwNI 113 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 879 CCTTAGG CC^T[ACGT]AGG
Asp700I 711 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 446 GGTCC G^G[AT]CC
BanI 704 GGCGCC G^G[CT][GA]CC
BbeI 704 GGCGCC GGCGC^C
BcgI 848 CGATTCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 806 CTAG C^TAG
BsaBI 141 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 704 GGCGCC G[GA]^CG[CT]C
BsaJI 759 CCATGG C^C[ACGT][ACGT]GG
BsmAI 414 GTCTC GTCTC(1/5)
BspLU11I 902 ACATGT A^CATGT
BssKI 75 CCAGG ^CC[ACGT]GG
Bst1107I 399 GTATAC GTA^TAC
BstNI 75 CCAGG CC^[AT]GG
BstZ17I 399 GTATAC GTA^TAC
Bsu36I 879 CCTTAGG CC^T[ACGT]AGG
Cac8I 316 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 705 GCGC GCG^C
Cfr13I 446 GGTCC G^G[ACGT]CC
DraII 445 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 759 CCATGG C^C[GA][CT]GG
EarI 431 CTCTTC CTCTTC(1/4)
Eco81I 879 CCTTAGG CC^T[ACGT]AGG
EcoO109I 445 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 75 CCAGG ^CC[AT]GG
EcoRV 892 GATATC GAT^ATC
EcoT14I 759 CCATGG C^C[AT][AT]GG
HaeII 704 GGCGCC [GA]GCGC^[CT]
HhaI 705 GCGC GCG^C
Hin1I 704 GGCGCC G[GA]^CG[CT]C
HinP1I 705 GCGC G^CGC
KasI 704 GGCGCC G^GCGCC
Ksp632I 431 CTCTTC CTCTTC(1/4)
MaeI 806 CTAG C^TAG
MamI 141 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 75 CCAGG CC^[AT]GG
MwoI 352 GCAGCGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 704 GGCGCC GG^CGCC
NcoI 759 CCATGG C^CATGG
NspI 902 ACATGT [GA]CATG^[CT]
PleI 155 GACTC GAGTC(4/5)
PpuMI 445 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 183 GTAC GT^AC
SapI 430 GCTCTTC GCTCTTC(1/4)
Sau96I 446 GGTCC G^G[ACGT]CC
ScrFI 75 CCAGG CC^[ACGT]GG
SfoI 704 GGCGCC GGC^GCC
StyI 759 CCATGG C^C[AT][AT]GG
XmnI 711 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccI 106 GTCTAC GT^[AC][GT]AC
399 GTATAC
AspHI 428 GTGCTC G[AT]GC[AT]^C
468 GTGCTC
BmyI 428 GTGCTC G[AGT]GC[ACT]^C
468 GTGCTC
BsiHKAI 428 GTGCTC G[AT]GC[AT]^C
468 GTGCTC
BsiYI 369 CCGTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
449 CCTATACATGG
BslI 369 CCGTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
449 CCTATACATGG
Bsp1286I 428 GTGCTC G[AGT]GC[ACT]^C
468 GTGCTC
DpnII 692 GATC ^GATC
870 GATC
HphI 627 GGTGA GGTGA(8/7)
923 TCACC
MboI 692 GATC ^GATC
870 GATC
MslI 117 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CACTATTATG
NdeII 692 GATC ^GATC
870 GATC
Sau3AI 692 GATC ^GATC
870 GATC
TfiI 514 GAATC G^A[AT]TC
849 GATTC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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BbvI DdeI Fnu4HI HinfI ItaI
MaeIII MboII MnlI NlaIII NlaIV
SfaNI SfcI TseI Tsp45I Tsp509I
TspRI