Restriction map for
pFN21AE5908
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1286I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI TfiI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
AfaI 765 GTAC GT^AC
AflII 157 CTTAAG C^TTAAG
AflIII 709 ACATGT A^C[GA][CT]GT
AvaII 748 GGACC G^G[AT]CC
BbsI 258 GAAGAC GAAGAC(2/6)
BfaI 230 CTAG C^TAG
BfrI 157 CTTAAG C^TTAAG
BglII 326 AGATCT A^GATCT
BpmI 322 CTGGAG CTGGAG(16/14)
BpuAI 258 GAAGAC GAAGAC(2/6)
BsaBI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 91 CGTCTC CGTCTC(1/5)
Bsp1407I 764 TGTACA T^GTACA
BspLU11I 709 ACATGT A^CATGT
BsrGI 764 TGTACA T^GTACA
BsrI 405 CCAGT ACTGG(1/-1)
BstXI 306 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 791 CCATGG C^C[GA][CT]GG
EcoT14I 791 CCATGG C^C[AT][AT]GG
HgaI 90 GCGTC GACGC(5/10)
HindIII 892 AAGCTT A^AGCTT
HpaI 577 GTTAAC GTT^AAC
MaeI 230 CTAG C^TAG
MamI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 607 GCCATTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 791 CCATGG C^CATGG
PflMI 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 765 GTAC GT^AC
SfaNI 244 GATGC GCATC(5/9)
SmlI 157 CTTAAG C^T[CT][GA]AG
SspBI 764 TGTACA T^GTACA
StyI 791 CCATGG C^C[AT][AT]GG
Van91I 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 405 CCAGTGGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 807 AGCT AG^CT
893 AGCT
BamHI 51 GGATCC G^GATCC
445 GGATCC
BbvI 537 GCAGC GCAGC(8/12)
317 GCTGC
BclI 512 TGATCA T^GATCA
911 TGATCA
BsaJI 437 CCTGGG C^C[ACGT][ACGT]GG
791 CCATGG
BsgI 382 CTGCAC GTGCAG(16/14)
614 GTGCAG
BsmAI 92 GTCTC GTCTC(1/5)
715 GTCTC
BsmI 456 GCATTC GAATGC(1/-1)
660 GCATTC
FbaI 512 TGATCA T^GATCA
911 TGATCA
Fnu4HI 317 GCTGC GC^[ACGT]GC
537 GCAGC
HincII 352 GTTGAC GT[CT]^[GA]AC
577 GTTAAC
HindII 352 GTTGAC GT[CT]^[GA]AC
577 GTTAAC
ItaI 317 GCTGC GC^[ACGT]GC
537 GCAGC
MaeIII 19 GTGAC ^GT[ACGT]AC
530 GTGAC
MseI 158 TTAA T^TAA
578 TTAA
MslI 723 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATATTTATG
NspI 709 ACATGT [GA]CATG^[CT]
769 ACATGC
SspI 69 AATATT AAT^ATT
636 AATATT
Tru9I 158 TTAA T^TAA
578 TTAA
TseI 317 GCTGC G^C[AT]GC
537 GCAGC
Tsp45I 19 GTGAC ^GT[GC]AC
530 GTGAC
Tsp509I 118 AATT ^AATT
908 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AlwI BsiYI BslI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII FokI HaeIII MboI MboII
MflI MnlI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TspRI
XhoII