Restriction map for
pFN21AE5491
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BglII BlnI
BpmI BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CfoI Cfr10I CpoI
DraI DraII DraIII DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MaeI
MfeI MflI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TseI Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AciI 523 GCGG CCGC(-3/-1)
AfaI 261 GTAC GT^AC
AflII 757 CTTAAG C^TTAAG
AhdI 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 393 GGATC GGATC(4/5)
AlwNI 241 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 145 CCTGAGG CC^T[ACGT]AGG
Asp700I 415 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 592 GGACC G^G[AT]CC
BanII 130 GGGCTC G[GA]GC[CT]^C
BciVI 627 GTATCC GTATCC(6/5)
BclI 482 TGATCA T^GATCA
BfrI 757 CTTAAG C^TTAAG
BlpI 1004 GCTTAGC GC^T[ACGT]AGC
BmyI 130 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 1004 GCTTAGC GC^T[ACGT]AGC
BsaBI 622 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 692 GAGGAG GAGGAG(10/8)
BsmI 954 GAATGC GAATGC(1/-1)
Bsp1286I 130 GGGCTC G[AGT]GC[ACT]^C
BspDI 446 ATCGAT AT^CGAT
BspHI 474 TCATGA T^CATGA
BspMI 209 ACCTGC ACCTGC(4/8)
BstF5I 806 CATCC GGATG(2/0)
Bsu36I 145 CCTGAGG CC^T[ACGT]AGG
CelII 1004 GCTTAGC GC^T[ACGT]AGC
ClaI 446 ATCGAT AT^CGAT
DrdI 59 GACACCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 593 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 470 CTCTTC CTCTTC(1/4)
Eco81I 145 CCTGAGG CC^T[ACGT]AGG
FbaI 482 TGATCA T^GATCA
Fnu4HI 523 GCGGC GC^[ACGT]GC
FokI 806 CATCC GGATG(9/13)
HincII 331 GTTGAC GT[CT]^[GA]AC
HindII 331 GTTGAC GT[CT]^[GA]AC
ItaI 523 GCGGC GC^[ACGT]GC
Ksp632I 470 CTCTTC CTCTTC(1/4)
MaeII 812 ACGT A^CGT
MamI 622 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 758 TTAA T^TAA
MwoI 697 GCGATTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 240 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 474 TCATGA T^CATGA
RsaI 261 GTAC GT^AC
SfaNI 805 GCATC GCATC(5/9)
SfcI 899 CTGTAG C^T[GA][CT]AG
SmlI 757 CTTAAG C^T[CT][GA]AG
TfiI 492 GAATC G^A[AT]TC
Tru9I 758 TTAA T^TAA
Van91I 240 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 236 CCAGCCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 415 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 25 AAATTT [GA]^AATT[CT]
115 GAATTT
ApoI 25 AAATTT [GA]^AATT[CT]
115 GAATTT
BalI 233 TGGCCA TGG^CCA
455 TGGCCA
BsaJI 245 CCCTGG C^C[ACGT][ACGT]GG
246 CCTGGG
BsiYI 240 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTTTCTGTGG
BslI 240 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
583 CCTTTCTGTGG
BsmFI 243 GTCCC GGGAC(10/14)
838 GTCCC
BsrI 667 ACTGG ACTGG(1/-1)
240 CCAGT
BssKI 246 CCTGG ^CC[ACGT]GG
527 CCTGG
BstNI 246 CCTGG CC^[AT]GG
527 CCTGG
Cac8I 235 GCCAGC GC[ACGT]^[ACGT]GC
646 GCTTGC
Cfr13I 592 GGACC G^G[ACGT]CC
863 GGCCC
EaeI 233 TGGCCA [CT]^GGCC[GA]
455 TGGCCA
EcoRII 246 CCTGG ^CC[AT]GG
527 CCTGG
HindIII 788 AAGCTT A^AGCTT
1002 AAGCTT
MluNI 233 TGGCCA TGG^CCA
455 TGGCCA
MscI 233 TGGCCA TGG^CCA
455 TGGCCA
MvaI 246 CCTGG CC^[AT]GG
527 CCTGG
Sau96I 592 GGACC G^G[ACGT]CC
863 GGCCC
ScrFI 246 CCTGG CC^[ACGT]GG
527 CCTGG
TaqI 447 TCGA T^CGA
498 TCGA
TthHB8I 447 TCGA T^CGA
498 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AluI DdeI DpnII HaeIII HinfI
HphI MaeIII MboI MboII MnlI
NdeII NlaIII NlaIV PleI Sau3AI
Tsp45I Tsp509I TspRI