Restriction map for
pFN21AE6017
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 702 GTCTAC GT^[AC][GT]AC
AvaII 499 GGTCC G^G[AT]CC
BamHI 51 GGATCC G^GATCC
BbsI 328 GTCTTC GAAGAC(2/6)
BbvCI 322 GCTGAGG CCTCAGC(-5/-2)
BcgI 406 CGACAGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 328 GTCTTC GAAGAC(2/6)
BseRI 926 GAGGAG GAGGAG(10/8)
BsgI 382 CTGCAC GTGCAG(16/14)
BsmFI 500 GTCCC GGGAC(10/14)
BstF5I 651 CATCC GGATG(2/0)
BstXI 306 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 51 GGATCC [GA]^GATC[CT]
FokI 651 CATCC GGATG(9/13)
HapII 716 CCGG C^CGG
HincII 906 GTTAAC GT[CT]^[GA]AC
HindII 906 GTTAAC GT[CT]^[GA]AC
HpaI 906 GTTAAC GTT^AAC
HpaII 716 CCGG C^CGG
MaeII 853 ACGT A^CGT
MflI 51 GGATCC [GA]^GATC[CT]
MspI 716 CCGG C^CGG
MwoI 710 GCAGCTCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI 708 CTGCAG CTGCA^G
SfaNI 202 GATGC GCATC(5/9)
SphI 553 GCATGC GCATG^C
SspI 16 AATATT AAT^ATT
StuI 576 AGGCCT AGG^CCT
TaqI 405 TCGA T^CGA
TfiI 402 GAATC G^A[AT]TC
TthHB8I 405 TCGA T^CGA
XhoII 51 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 25 GAATTT [GA]^AATT[CT]
861 AAATTC
AlwI 52 GATCC GGATC(4/5)
51 GGATC
ApoI 25 GAATTT [GA]^AATT[CT]
861 AAATTC
BalI 365 TGGCCA TGG^CCA
434 TGGCCA
BbvI 710 GCAGC GCAGC(8/12)
205 GCTGC
BsmAI 3 GAGAC GTCTC(1/5)
485 GTCTC
Bsp1407I 453 TGTACA T^GTACA
479 TGTACA
BspHI 398 TCATGA T^CATGA
800 TCATGA
BspMI 119 ACCTGC ACCTGC(4/8)
706 ACCTGC
BsrGI 453 TGTACA T^GTACA
479 TGTACA
BsrI 543 ACTGG ACTGG(1/-1)
549 ACTGG
BssKI 36 CCTGG ^CC[ACGT]GG
55 CCTGG
BstNI 36 CCTGG CC^[AT]GG
55 CCTGG
Cfr13I 288 GGGCC G^G[ACGT]CC
499 GGTCC
DpnII 52 GATC ^GATC
355 GATC
EaeI 365 TGGCCA [CT]^GGCC[GA]
434 TGGCCA
EcoRII 36 CCTGG ^CC[AT]GG
55 CCTGG
EcoT14I 155 CCTTGG C^C[AT][AT]GG
276 CCAAGG
Fnu4HI 205 GCTGC GC^[ACGT]GC
710 GCAGC
HphI 102 GGTGA GGTGA(8/7)
342 GGTGA
ItaI 205 GCTGC GC^[ACGT]GC
710 GCAGC
MaeIII 103 GTGAC ^GT[ACGT]AC
527 GTGAC
MboI 52 GATC ^GATC
355 GATC
MboII 176 TCTTC GAAGA(8/7)
329 TCTTC
MluNI 365 TGGCCA TGG^CCA
434 TGGCCA
MscI 365 TGGCCA TGG^CCA
434 TGGCCA
MvaI 36 CCTGG CC^[AT]GG
55 CCTGG
NdeII 52 GATC ^GATC
355 GATC
PleI 647 GAGTC GAGTC(4/5)
837 GAGTC
RcaI 398 TCATGA T^CATGA
800 TCATGA
Sau3AI 52 GATC ^GATC
355 GATC
Sau96I 288 GGGCC G^G[ACGT]CC
499 GGTCC
ScrFI 36 CCTGG CC^[ACGT]GG
55 CCTGG
SfcI 708 CTGCAG C^T[GA][CT]AG
881 CTATAG
SspBI 453 TGTACA T^GTACA
479 TGTACA
StyI 155 CCTTGG C^C[AT][AT]GG
276 CCAAGG
TseI 205 GCTGC G^C[AT]GC
710 GCAGC
Tsp45I 103 GTGAC ^GT[GC]AC
527 GTGAC
XcmI 146 CCAGCCCTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
306 CCATTTCTTTGGTGG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
Cac8I DdeI HaeIII HinfI MnlI
MseI MslI NlaIII NlaIV NspI
RsaI Tru9I Tsp509I TspRI