Restriction map for
pFN21AE5375
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI KasI
KpnI KspI MaeII MamI MluI
MroI MseI MspA1I MspI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 813 CGCG CG^CG
AlwI 816 GATCC GGATC(4/5)
ApaI 730 GGGCCC GGGCC^C
BalI 347 TGGCCA TGG^CCA
BanI 586 GGCACC G^G[CT][GA]CC
BbsI 739 GTCTTC GAAGAC(2/6)
BbvI 263 GCTGC GCAGC(8/12)
BfaI 517 CTAG C^TAG
BpmI 657 CTGGAG CTGGAG(16/14)
BpuAI 739 GTCTTC GAAGAC(2/6)
BsaJI 138 CCTTGG C^C[ACGT][ACGT]GG
BseRI 195 CTCCTC GAGGAG(10/8)
BsgI 43 GTGCAG GTGCAG(16/14)
BsmAI 894 GAGAC GTCTC(1/5)
BsmFI 699 GTCCC GGGAC(10/14)
BsmI 438 GCATTC GAATGC(1/-1)
Bsp1407I 77 TGTACA T^GTACA
BsrGI 77 TGTACA T^GTACA
BsrI 386 ACTGG ACTGG(1/-1)
BstF5I 623 CATCC GGATG(2/0)
BstUI 813 CGCG CG^CG
DraIII 340 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 347 TGGCCA [CT]^GGCC[GA]
EcoRI 7 GAATTC G^AATTC
EcoT14I 138 CCTTGG C^C[AT][AT]GG
EcoT22I 436 ATGCAT ATGCA^T
Fnu4HI 263 GCTGC GC^[ACGT]GC
FokI 623 CATCC GGATG(9/13)
HgaI 737 GCGTC GACGC(5/10)
ItaI 263 GCTGC GC^[ACGT]GC
MaeI 517 CTAG C^TAG
MaeIII 512 GTGAC ^GT[ACGT]AC
MfeI 86 CAATTG C^AATTG
MluNI 347 TGGCCA TGG^CCA
MscI 347 TGGCCA TGG^CCA
MunI 86 CAATTG C^AATTG
MvnI 813 CGCG CG^CG
NsiI 436 ATGCAT ATGCA^T
PflMI 339 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 660 GAGCTC GAGCT^C
SapI 474 GCTCTTC GCTCTTC(1/4)
SfaNI 622 GCATC GCATC(5/9)
SfcI 203 CTACAG C^T[GA][CT]AG
SspBI 77 TGTACA T^GTACA
StuI 754 AGGCCT AGG^CCT
StyI 138 CCTTGG C^C[AT][AT]GG
TseI 263 GCTGC G^C[AT]GC
Tsp45I 512 GTGAC ^GT[GC]AC
Van91I 339 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 773 CCATAGACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 200 CCGC CCGC(-3/-1)
812 CCGC
AcsI 7 GAATTC [GA]^AATT[CT]
99 GAATTT
ApoI 7 GAATTC [GA]^AATT[CT]
99 GAATTT
AspHI 112 GTGCTC G[AT]GC[AT]^C
660 GAGCTC
AvaII 612 GGTCC G^G[AT]CC
698 GGTCC
BanII 660 GAGCTC G[GA]GC[CT]^C
730 GGGCCC
BbvCI 162 CCTCAGC CCTCAGC(-5/-2)
363 CCTCAGC
BclI 494 TGATCA T^GATCA
599 TGATCA
BglII 185 AGATCT A^GATCT
225 AGATCT
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
660 GAGCTC
BspHI 122 TCATGA T^CATGA
380 TCATGA
BspMI 470 ACCTGC ACCTGC(4/8)
390 GCAGGT
BssKI 36 CCAGG ^CC[ACGT]GG
656 CCTGG
BstNI 36 CCAGG CC^[AT]GG
656 CCTGG
BstYI 185 AGATCT [GA]^GATC[CT]
225 AGATCT
EarI 475 CTCTTC CTCTTC(1/4)
870 GAAGAG
EcoRII 36 CCAGG ^CC[AT]GG
656 CCTGG
FbaI 494 TGATCA T^GATCA
599 TGATCA
Ksp632I 475 CTCTTC CTCTTC(1/4)
870 GAAGAG
MflI 185 AGATCT [GA]^GATC[CT]
225 AGATCT
MvaI 36 CCAGG CC^[AT]GG
656 CCTGG
PpuMI 611 GGGTCCT [GA]G^G[AT]CC[CT]
697 GGGTCCC
RcaI 122 TCATGA T^CATGA
380 TCATGA
ScrFI 36 CCAGG CC^[ACGT]GG
656 CCTGG
XhoII 185 AGATCT [GA]^GATC[CT]
225 AGATCT
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI BmyI BsiYI BslI
Bsp1286I Cac8I Cfr13I DdeI DpnII
DraII EcoO109I HaeIII MboI MboII
MnlI MslI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I Tsp509I TspRI