Restriction map for
pFN21AE5809
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI KspI MamI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1361 CGCG CG^CG
AcyI 1919 GACGCC G[GA]^CG[CT]C
AlwNI 88 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 162 CCTGAGG CC^T[ACGT]AGG
AseI 2668 ATTAAT AT^TAAT
AsnI 2668 ATTAAT AT^TAAT
BalI 762 TGGCCA TGG^CCA
BbsI 352 GAAGAC GAAGAC(2/6)
BciVI 540 GTATCC GTATCC(6/5)
BlpI 962 GCTTAGC GC^T[ACGT]AGC
BpmI 1808 CTGGAG CTGGAG(16/14)
Bpu1102I 962 GCTTAGC GC^T[ACGT]AGC
BpuAI 352 GAAGAC GAAGAC(2/6)
BsaHI 1919 GACGCC G[GA]^CG[CT]C
BsmBI 1917 GAGACG CGTCTC(1/5)
BsmI 1317 GAATGC GAATGC(1/-1)
BspLU11I 2520 ACATGT A^CATGT
BstAPI 2227 GCAACTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1179 GGTCACC G^GT[ACGT]ACC
BstPI 1179 GGTCACC G^GT[ACGT]ACC
BstUI 1361 CGCG CG^CG
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
CelII 962 GCTTAGC GC^T[ACGT]AGC
DraI 1732 TTTAAA TTT^AAA
DraII 1235 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1369 CCATGG C^C[GA][CT]GG
EaeI 762 TGGCCA [CT]^GGCC[GA]
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
EcoNI 2314 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1235 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1179 GGTCACC G^GT[ACGT]ACC
EcoT22I 1493 ATGCAT ATGCA^T
FauI 476 CCCGC CCCGC(4/6)
Hin1I 1919 GACGCC G[GA]^CG[CT]C
HincII 1143 GTCAAC GT[CT]^[GA]AC
HindII 1143 GTCAAC GT[CT]^[GA]AC
HindIII 1515 AAGCTT A^AGCTT
MfeI 1837 CAATTG C^AATTG
MluI 1360 ACGCGT A^CGCGT
MluNI 762 TGGCCA TGG^CCA
MscI 762 TGGCCA TGG^CCA
MunI 1837 CAATTG C^AATTG
MvnI 1361 CGCG CG^CG
NcoI 1369 CCATGG C^CATGG
NsiI 1493 ATGCAT ATGCA^T
PpuMI 1235 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2668 ATTAAT AT^TAAT
SapI 591 GCTCTTC GCTCTTC(1/4)
ScaI 2015 AGTACT AGT^ACT
SexAI 1238 ACCTGGT A^CC[AT]GGT
SnaBI 1720 TACGTA TAC^GTA
XcmI 1889 CCATTTATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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Asp700I 1674 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2666 GAATTAATTC
AspHI 589 GAGCTC G[AT]GC[AT]^C
2638 GAGCTC
BclI 1049 TGATCA T^GATCA
1068 TGATCA
BcnI 9 CCCGG CC^[GC]GG
1856 CCGGG
BfaI 1475 CTAG C^TAG
2275 CTAG
BsaAI 1101 TACGTG [CT]AC^GT[GA]
1720 TACGTA
BsaI 106 GAGACC GGTCTC(1/5)
1278 GAGACC
BseRI 1526 CTCCTC GAGGAG(10/8)
2078 CTCCTC
BsgI 563 CTGCAC GTGCAG(16/14)
2378 CTGCAC
BsiHKAI 589 GAGCTC G[AT]GC[AT]^C
2638 GAGCTC
BsmFI 362 GGGAC GGGAC(10/14)
1235 GGGAC
BspHI 1974 TCATGA T^CATGA
2696 TCATGA
BspMI 1506 GCAGGT ACCTGC(4/8)
2411 GCAGGT
BsrI 1221 ACTGG ACTGG(1/-1)
1424 CCAGT
BstYI 12 GGATCT [GA]^GATC[CT]
395 AGATCC
DraIII 527 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1061 CACTCTGTG
EcoRV 1605 GATATC GAT^ATC
2429 GATATC
FbaI 1049 TGATCA T^GATCA
1068 TGATCA
HapII 10 CCGG C^CGG
1856 CCGG
HgaI 277 GACGC GACGC(5/10)
1919 GACGC
HpaII 10 CCGG C^CGG
1856 CCGG
MaeI 1475 CTAG C^TAG
2275 CTAG
MflI 12 GGATCT [GA]^GATC[CT]
395 AGATCC
MspI 10 CCGG C^CGG
1856 CCGG
NciI 9 CCCGG CC^[GC]GG
1856 CCGGG
RcaI 1974 TCATGA T^CATGA
2696 TCATGA
SacI 589 GAGCTC GAGCT^C
2638 GAGCTC
SfcI 953 CTGTAG C^T[GA][CT]AG
2156 CTGTAG
SmlI 337 CTTGAG C^T[CT][GA]AG
663 CTCAAG
XhoII 12 GGATCT [GA]^GATC[CT]
395 AGATCC
XmnI 1674 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2666 GAATTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I