Restriction map for
pFN21AB7941
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI MaeIII MfeI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 35 GGTACC G^GTACC
AflIII 875 ACGCGT A^C[GA][CT]GT
Asp700I 591 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 35 GGTACC G^GTACC
BbsI 559 GTCTTC GAAGAC(2/6)
BbvCI 792 CCTCAGC CCTCAGC(-5/-2)
BglII 451 AGATCT A^GATCT
BpuAI 559 GTCTTC GAAGAC(2/6)
BsaBI 132 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 571 GTGCAG GTGCAG(16/14)
BsiEI 627 CGACCG CG[GA][CT]^CG
BsmAI 310 GAGAC GTCTC(1/5)
BsmBI 310 GAGACG CGTCTC(1/5)
BsrI 78 CCAGT ACTGG(1/-1)
BssHII 520 GCGCGC G^CGCGC
BstAPI 756 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 424 TTCGAA TT^CGAA
BstXI 110 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 117 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 716 CCAAGG C^C[AT][AT]GG
HaeII 582 GGCGCT [GA]GCGC^[CT]
KpnI 35 GGTACC GGTAC^C
KspI 907 CCGCGG CCGC^GG
MamI 132 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 875 ACGCGT A^CGCGT
NruI 750 TCGCGA TCG^CGA
NspV 424 TTCGAA TT^CGAA
PaeR7I 945 CTCGAG C^TCGAG
PflMI 858 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 664 GACTC GAGTC(4/5)
SacII 907 CCGCGG CCGC^GG
SfaNI 137 GCATC GCATC(5/9)
SfuI 424 TTCGAA TT^CGAA
SmlI 945 CTCGAG C^T[CT][GA]AG
SphI 542 GCATGC GCATG^C
StyI 716 CCAAGG C^C[AT][AT]GG
TfiI 979 GATTC G^A[AT]TC
Van91I 858 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 945 CTCGAG C^TCGAG
XmnI 591 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 36 GTAC GT^AC
612 GTAC
AlwI 132 GATCC GGATC(4/5)
126 GGATC
AlwNI 757 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
892 CAGCACCTG
BanI 35 GGTACC G^G[CT][GA]CC
483 GGTGCC
BfaI 41 CTAG C^TAG
737 CTAG
BstYI 126 GGATCT [GA]^GATC[CT]
451 AGATCT
DdeI 793 CTCAG C^T[ACGT]AG
975 CTCAG
DsaI 212 CCACGG C^C[GA][CT]GG
907 CCGCGG
EarI 826 GAAGAG CTCTTC(1/4)
991 GAAGAG
HinfI 664 GACTC G^A[ACGT]TC
979 GATTC
Ksp632I 826 GAAGAG CTCTTC(1/4)
991 GAAGAG
MaeI 41 CTAG C^TAG
737 CTAG
MflI 126 GGATCT [GA]^GATC[CT]
451 AGATCT
NspI 185 GCATGT [GA]CATG^[CT]
542 GCATGC
RsaI 36 GTAC GT^AC
612 GTAC
SfiI 207 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
805 GGCCAGAAGGGCC
XhoII 126 GGATCT [GA]^GATC[CT]
451 AGATCT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SmaI TaqI TseI TthHB8I XcmI
XmaCI XmaI