Restriction map for
pFN21AE5387
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT14I
FauI FbaI FseI HaeII HapII
HgaI Hin1I HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 326 GTAC GT^AC
AocI 235 CCTCAGG CC^T[ACGT]AGG
AvaII 446 GGACC G^G[AT]CC
AviII 289 TGCGCA TGC^GCA
BalI 590 TGGCCA TGG^CCA
BbsI 789 GTCTTC GAAGAC(2/6)
BciVI 646 GTATCC GTATCC(6/5)
BfaI 244 CTAG C^TAG
BpuAI 789 GTCTTC GAAGAC(2/6)
BsaJI 504 CCGAGG C^C[ACGT][ACGT]GG
BseRI 619 CTCCTC GAGGAG(10/8)
BsgI 545 CTGCAC GTGCAG(16/14)
BsmFI 45 GGGAC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BspMI 251 ACCTGC ACCTGC(4/8)
Bsu36I 235 CCTCAGG CC^T[ACGT]AGG
Cac8I 429 GCTGGC GC[ACGT]^[ACGT]GC
DraII 539 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 780 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 235 CCTCAGG CC^T[ACGT]AGG
EcoO109I 539 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 31 GAATTC G^AATTC
EcoT22I 578 ATGCAT ATGCA^T
FspI 289 TGCGCA TGC^GCA
MaeI 244 CTAG C^TAG
MboII 790 TCTTC GAAGA(8/7)
MluNI 590 TGGCCA TGG^CCA
MscI 590 TGGCCA TGG^CCA
MseI 848 TTAA T^TAA
NsiI 578 ATGCAT ATGCA^T
PflMI 729 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 404 TCATGA T^CATGA
RsaI 326 GTAC GT^AC
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 861 CTATAG C^T[GA][CT]AG
SmlI 703 CTCAAG C^T[CT][GA]AG
SpeI 243 ACTAGT A^CTAGT
TaqI 297 TCGA T^CGA
Tru9I 848 TTAA T^TAA
TthHB8I 297 TCGA T^CGA
Van91I 729 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 449 CCAGTGGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 100 GTCTAC GT^[AC][GT]AC
472 GTAGAC
AcsI 31 GAATTC [GA]^AATT[CT]
915 GAATTT
AlwI 439 GGATC GGATC(4/5)
757 GGATC
ApoI 31 GAATTC [GA]^AATT[CT]
915 GAATTT
BsmI 277 GCATTC GAATGC(1/-1)
455 GCATTC
BsrDI 273 CATTGC GCAATG(2/0)
519 CATTGC
BsrI 330 CCAGT ACTGG(1/-1)
449 CCAGT
BssKI 105 CCTGG ^CC[ACGT]GG
410 CCTGG
BstF5I 563 CATCC GGATG(2/0)
879 GGATG
BstNI 105 CCTGG CC^[AT]GG
410 CCTGG
CfoI 290 GCGC GCG^C
335 GCGC
EaeI 590 TGGCCA [CT]^GGCC[GA]
654 TGGCCG
EcoRII 105 CCTGG ^CC[AT]GG
410 CCTGG
FokI 563 CATCC GGATG(9/13)
879 GGATG
HhaI 290 GCGC GCG^C
335 GCGC
HinP1I 290 GCGC G^CGC
335 GCGC
HincII 151 GTTGAC GT[CT]^[GA]AC
687 GTCAAC
HindII 151 GTTGAC GT[CT]^[GA]AC
687 GTCAAC
MspA1I 73 CAGCTG C[AC]G^C[GT]G
427 CAGCTG
MvaI 105 CCTGG CC^[AT]GG
410 CCTGG
MwoI 337 GCCCTTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
509 GCAGTAACAGC
NlaIV 540 GGCCCC GG[ACGT]^[ACGT]CC
724 GGCTCC
PvuII 73 CAGCTG CAG^CTG
427 CAGCTG
ScrFI 105 CCTGG CC^[ACGT]GG
410 CCTGG
SfaNI 562 GCATC GCATC(5/9)
580 GCATC
TfiI 615 GATTC G^A[AT]TC
639 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmAI
Cfr13I DdeI DpnII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MnlI
MslI NdeII NlaIII PleI Sau3AI
Sau96I TseI Tsp45I Tsp509I TspRI