Restriction map for
pFN21AE5987
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MaeIII MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 628 GTAGAC GT^[AC][GT]AC
AciI 789 CCGC CCGC(-3/-1)
AflII 706 CTTAAG C^TTAAG
AluI 704 AGCT AG^CT
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
AspHI 611 GAGCAC G[AT]GC[AT]^C
BcnI 797 CCCGG CC^[GC]GG
BfrI 706 CTTAAG C^TTAAG
BpmI 334 CTGGAG CTGGAG(16/14)
BsaBI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 740 GTGCAG GTGCAG(16/14)
BsiHKAI 611 GAGCAC G[AT]GC[AT]^C
BsiYI 431 CCCAAATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 431 CCCAAATAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 557 TCATGA T^CATGA
BspMI 758 ACCTGC ACCTGC(4/8)
BsrDI 642 CATTGC GCAATG(2/0)
BstYI 83 GGATCT [GA]^GATC[CT]
EaeI 776 TGGCCG [CT]^GGCC[GA]
EcoRI 307 GAATTC G^AATTC
HapII 798 CCGG C^CGG
HincII 926 GTCAAC GT[CT]^[GA]AC
HindII 926 GTCAAC GT[CT]^[GA]AC
HpaII 798 CCGG C^CGG
HphI 32 TCACC GGTGA(8/7)
MamI 84 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 869 CAATTG C^AATTG
MflI 83 GGATCT [GA]^GATC[CT]
MspI 798 CCGG C^CGG
MunI 869 CAATTG C^AATTG
NciI 797 CCCGG CC^[GC]GG
NdeI 20 CATATG CA^TATG
PleI 337 GAGTC GAGTC(4/5)
PshAI 414 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 817 ATTAAT AT^TAAT
PstI 99 CTGCAG CTGCA^G
RcaI 557 TCATGA T^CATGA
ScaI 330 AGTACT AGT^ACT
SfcI 99 CTGCAG C^T[GA][CT]AG
SphI 461 GCATGC GCATG^C
XhoII 83 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 278 AAATTT [GA]^AATT[CT]
307 GAATTC
AlwI 663 GATCC GGATC(4/5)
83 GGATC
ApoI 278 AAATTT [GA]^AATT[CT]
307 GAATTC
BanII 160 GAGCCC G[GA]GC[CT]^C
175 GAGCCC
BbvI 101 GCAGC GCAGC(8/12)
702 GCAGC
BclI 353 TGATCA T^GATCA
746 TGATCA
BsaJI 58 CCAGGG C^C[ACGT][ACGT]GG
302 CCCAGG
BsmAI 542 GTCTC GTCTC(1/5)
805 GTCTC
BsrI 333 ACTGG ACTGG(1/-1)
678 ACTGG
Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC
461 GCATGC
FbaI 353 TGATCA T^GATCA
746 TGATCA
Fnu4HI 101 GCAGC GC^[ACGT]GC
702 GCAGC
HaeIII 567 GGCC GG^CC
777 GGCC
ItaI 101 GCAGC GC^[ACGT]GC
702 GCAGC
MwoI 162 GCCCTCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
292 GCCTGCTTTGC
TfiI 323 GATTC G^A[AT]TC
371 GATTC
TseI 101 GCAGC G^C[AT]GC
702 GCAGC
XcmI 35 CCACCTTCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
764 CCATCATCAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI BfaI BmyI Bsp1286I BssKI
BstF5I BstNI DdeI DpnII EcoRII
FokI HinfI MaeI MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NspI RsaI Sau3AI ScrFI
SfaNI SmlI Tru9I Tsp509I TspRI