Restriction map for
pFN21AE6077
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 124 GTCTAC GT^[AC][GT]AC
Alw44I 89 GTGCAC G^TGCAC
Aor51HI 423 AGCGCT AGC^GCT
ApaLI 89 GTGCAC G^TGCAC
BanI 151 GGCACC G^G[CT][GA]CC
BbvCI 305 CCTCAGC CCTCAGC(-5/-2)
BglI 677 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 484 GGTCTC GGTCTC(1/5)
BsmAI 485 GTCTC GTCTC(1/5)
BsmFI 298 GTCCC GGGAC(10/14)
CfoI 424 GCGC GCG^C
DraII 539 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 638 CCATGG C^C[GA][CT]GG
Eco47III 423 AGCGCT AGC^GCT
EcoNI 531 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 539 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 638 CCATGG C^C[AT][AT]GG
FauI 181 CCCGC CCCGC(4/6)
HaeII 423 AGCGCT [GA]GCGC^[CT]
HhaI 424 GCGC GCG^C
HinP1I 424 GCGC G^CGC
HincII 3 GTCAAC GT[CT]^[GA]AC
HindII 3 GTCAAC GT[CT]^[GA]AC
HindIII 596 AAGCTT A^AGCTT
HphI 431 TCACC GGTGA(8/7)
MaeIII 244 GTCAC ^GT[ACGT]AC
MseI 803 TTAA T^TAA
NcoI 638 CCATGG C^CATGG
PflMI 155 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 539 AGGTCCT [GA]G^G[AT]CC[CT]
SphI 71 GCATGC GCATG^C
StuI 78 AGGCCT AGG^CCT
StyI 638 CCATGG C^C[AT][AT]GG
TaqI 474 TCGA T^CGA
Tru9I 803 TTAA T^TAA
Tsp45I 244 GTCAC ^GT[GC]AC
Tsp509I 444 AATT ^AATT
TthHB8I 474 TCGA T^CGA
Van91I 155 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 53 CCACCTTCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 182 CCGC CCGC(-3/-1)
192 CCGC
AluI 597 AGCT AG^CT
815 AGCT
AvaII 540 GGTCC G^G[AT]CC
648 GGACC
BalI 395 TGGCCA TGG^CCA
461 TGGCCA
BseRI 427 CTCCTC GAGGAG(10/8)
766 CTCCTC
BsrI 141 ACTGG ACTGG(1/-1)
393 ACTGG
EaeI 395 TGGCCA [CT]^GGCC[GA]
461 TGGCCA
HinfI 355 GAGTC G^A[ACGT]TC
706 GAGTC
MluNI 395 TGGCCA TGG^CCA
461 TGGCCA
MscI 395 TGGCCA TGG^CCA
461 TGGCCA
MslI 39 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
565 CATCCAGATG
NspI 71 GCATGC [GA]CATG^[CT]
518 ACATGC
PleI 355 GAGTC GAGTC(4/5)
706 GAGTC
PstI 175 CTGCAG CTGCA^G
758 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AlwI AspHI BbvI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BsrDI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII ItaI
Ksp632I MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI