Restriction map for
pFN21AE6021
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tru9I Tsp45I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 727 ACACGT A^C[GA][CT]GT
AocI 695 CCTCAGG CC^T[ACGT]AGG
AspI 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 815 CCCGAG C^[CT]CG[GA]G
BbvI 465 GCTGC GCAGC(8/12)
BclI 431 TGATCA T^GATCA
BglI 199 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 67 CTGGAG CTGGAG(16/14)
BsiEI 425 CGGTCG CG[GA][CT]^CG
BsoBI 815 CCCGAG C^[CT]CG[GA]G
Bsp1407I 822 TGTACA T^GTACA
BspHI 276 TCATGA T^CATGA
BsrFI 204 GCCGGC [GA]^CCGG[CT]
BsrGI 822 TGTACA T^GTACA
Bsu36I 695 CCTCAGG CC^T[ACGT]AGG
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
EarI 24 CTCTTC CTCTTC(1/4)
Eco81I 695 CCTCAGG CC^T[ACGT]AGG
EcoNI 300 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 921 CCAAGG C^C[AT][AT]GG
FauI 707 CCCGC CCCGC(4/6)
FbaI 431 TGATCA T^GATCA
Fnu4HI 465 GCTGC GC^[ACGT]GC
HapII 205 CCGG C^CGG
HindIII 716 AAGCTT A^AGCTT
HpaII 205 CCGG C^CGG
ItaI 465 GCTGC GC^[ACGT]GC
Ksp632I 24 CTCTTC CTCTTC(1/4)
MspI 205 CCGG C^CGG
NaeI 204 GCCGGC GCC^GGC
NgoMIV 204 GCCGGC G^CCGGC
NlaIV 442 GGGACC GG[ACGT]^[ACGT]CC
PflFI 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 257 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 340 GAGTC GAGTC(4/5)
RcaI 276 TCATGA T^CATGA
SapI 23 GCTCTTC GCTCTTC(1/4)
SexAI 218 ACCTGGT A^CC[AT]GGT
SfiI 198 GGCCATGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 822 TGTACA T^GTACA
StuI 63 AGGCCT AGG^CCT
StyI 921 CCAAGG C^C[AT][AT]GG
TseI 465 GCTGC G^C[AT]GC
Tsp509I 159 AATT ^AATT
TspRI 213 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 257 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 708 CCGC CCGC(-3/-1)
795 CCGC
AspHI 349 GTGCTC G[AT]GC[AT]^C
547 GAGCAC
AvaII 222 GGTCC G^G[AT]CC
443 GGACC
BfaI 407 CTAG C^TAG
500 CTAG
BsiHKAI 349 GTGCTC G[AT]GC[AT]^C
547 GAGCAC
BsmFI 442 GGGAC GGGAC(10/14)
908 GGGAC
DpnII 432 GATC ^GATC
903 GATC
MaeI 407 CTAG C^TAG
500 CTAG
MboI 432 GATC ^GATC
903 GATC
NdeII 432 GATC ^GATC
903 GATC
Sau3AI 432 GATC ^GATC
903 GATC
TfiI 56 GAATC G^A[AT]TC
282 GATTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI BalI BanII BmyI
BsaAI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
FokI HaeIII HinfI HphI MaeII
MboII MluNI MnlI MscI MslI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI SfaNI XcmI