Restriction map for
pFN21AE5964
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbvCI BcgI BclI BcnI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
Cac8I CfoI Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI HphI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 841 GTATAC GT^[AC][GT]AC
AciI 903 CCGC CCGC(-3/-1)
AcsI 28 AAATTT [GA]^AATT[CT]
AflII 493 CTTAAG C^TTAAG
AflIII 454 ACACGT A^C[GA][CT]GT
AluI 292 AGCT AG^CT
ApoI 28 AAATTT [GA]^AATT[CT]
AseI 603 ATTAAT AT^TAAT
AsnI 603 ATTAAT AT^TAAT
AspHI 862 GTGCTC G[AT]GC[AT]^C
BalI 377 TGGCCA TGG^CCA
BciVI 254 GTATCC GTATCC(6/5)
BfaI 64 CTAG C^TAG
BfrI 493 CTTAAG C^TTAAG
BlpI 771 GCTGAGC GC^T[ACGT]AGC
BmyI 862 GTGCTC G[AGT]GC[ACT]^C
BpmI 341 CTGGAG CTGGAG(16/14)
Bpu1102I 771 GCTGAGC GC^T[ACGT]AGC
BsaJI 782 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 862 GTGCTC G[AT]GC[AT]^C
Bsp1286I 862 GTGCTC G[AGT]GC[ACT]^C
BspMI 845 ACCTGC ACCTGC(4/8)
BsrDI 114 CATTGC GCAATG(2/0)
BsrFI 49 ACCGGC [GA]^CCGG[CT]
BsrI 625 ACTGG ACTGG(1/-1)
BssKI 58 CCTGG ^CC[ACGT]GG
Bst1107I 841 GTATAC GTA^TAC
BstNI 58 CCTGG CC^[AT]GG
BstXI 209 CCACTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 83 GGATCT [GA]^GATC[CT]
BstZ17I 841 GTATAC GTA^TAC
CelII 771 GCTGAGC GC^T[ACGT]AGC
Cfr10I 49 ACCGGC [GA]^CCGG[CT]
EaeI 377 TGGCCA [CT]^GGCC[GA]
EarI 193 CTCTTC CTCTTC(1/4)
EcoRII 58 CCTGG ^CC[AT]GG
EcoT14I 782 CCTTGG C^C[AT][AT]GG
EcoT22I 642 ATGCAT ATGCA^T
HapII 50 CCGG C^CGG
HpaII 50 CCGG C^CGG
Ksp632I 193 CTCTTC CTCTTC(1/4)
MaeI 64 CTAG C^TAG
MflI 83 GGATCT [GA]^GATC[CT]
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MspI 50 CCGG C^CGG
MvaI 58 CCTGG CC^[AT]GG
NsiI 642 ATGCAT ATGCA^T
PshBI 603 ATTAAT AT^TAAT
ScaI 465 AGTACT AGT^ACT
ScrFI 58 CCTGG CC^[ACGT]GG
SfaNI 593 GCATC GCATC(5/9)
SmlI 493 CTTAAG C^T[CT][GA]AG
SspI 471 AATATT AAT^ATT
StyI 782 CCTTGG C^C[AT][AT]GG
TaqI 878 TCGA T^CGA
Tsp45I 386 GTGAC ^GT[GC]AC
TthHB8I 878 TCGA T^CGA
XhoII 83 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 399 GTAC GT^AC
466 GTAC
AlwI 138 GATCC GGATC(4/5)
83 GGATC
AlwNI 206 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
720 CAGCACCTG
BbrPI 272 CACGTG CAC^GTG
455 CACGTG
BbsI 440 GTCTTC GAAGAC(2/6)
715 GTCTTC
BbvI 293 GCTGC GCAGC(8/12)
924 GCTGC
BpuAI 440 GTCTTC GAAGAC(2/6)
715 GTCTTC
BsaAI 272 CACGTG [CT]AC^GT[GA]
455 CACGTG
BsmAI 167 GTCTC GTCTC(1/5)
730 GTCTC
BsmI 519 GAATGC GAATGC(1/-1)
53 GCATTC
Fnu4HI 293 GCTGC GC^[ACGT]GC
924 GCTGC
HaeIII 61 GGCC GG^CC
378 GGCC
HincII 45 GTTGAC GT[CT]^[GA]AC
240 GTCAAC
HindII 45 GTTGAC GT[CT]^[GA]AC
240 GTCAAC
ItaI 293 GCTGC GC^[ACGT]GC
924 GCTGC
MaeII 273 ACGT A^CGT
456 ACGT
MaeIII 386 GTGAC ^GT[ACGT]AC
542 GTTAC
NspI 568 GCATGT [GA]CATG^[CT]
767 ACATGC
PleI 67 GAGTC GAGTC(4/5)
165 GAGTC
PmaCI 272 CACGTG CAC^GTG
455 CACGTG
PmlI 272 CACGTG CAC^GTG
455 CACGTG
RsaI 399 GTAC GT^AC
466 GTAC
TseI 293 GCTGC G^C[AT]GC
924 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BsiYI BslI BstF5I DdeI DpnII
FokI HinfI MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
Sau3AI SfcI TfiI Tru9I Tsp509I
TspRI