Restriction map for
pFN21AE6052
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BbeI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflII 673 CTTAAG C^TTAAG
AflIII 694 ACACGT A^C[GA][CT]GT
BamHI 9 GGATCC G^GATCC
BanI 477 GGCACC G^G[CT][GA]CC
BbrPI 695 CACGTG CAC^GTG
BcnI 585 CCGGG CC^[GC]GG
BfaI 127 CTAG C^TAG
BfrI 673 CTTAAG C^TTAAG
BsaAI 695 CACGTG [CT]AC^GT[GA]
BsgI 467 CTGCAC GTGCAG(16/14)
BsmAI 730 GTCTC GTCTC(1/5)
BsmBI 729 CGTCTC CGTCTC(1/5)
BspHI 560 TCATGA T^CATGA
BsrBI 516 CCGCTC CCGCTC(-3/-3)
BsrDI 20 GCAATG GCAATG(2/0)
BssSI 425 CTCGTG CACGAG(-5/-1)
BstAPI 165 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 9 GGATCC [GA]^GATC[CT]
DpnII 10 GATC ^GATC
EarI 70 GAAGAG CTCTTC(1/4)
EcoRV 580 GATATC GAT^ATC
FauI 459 GCGGG CCCGC(4/6)
HapII 585 CCGG C^CGG
HgaI 728 GCGTC GACGC(5/10)
HpaII 585 CCGG C^CGG
Ksp632I 70 GAAGAG CTCTTC(1/4)
MaeI 127 CTAG C^TAG
MboI 10 GATC ^GATC
MfeI 872 CAATTG C^AATTG
MflI 9 GGATCC [GA]^GATC[CT]
MseI 674 TTAA T^TAA
MspI 585 CCGG C^CGG
MunI 872 CAATTG C^AATTG
NciI 585 CCGGG CC^[GC]GG
NdeII 10 GATC ^GATC
NspI 200 GCATGC [GA]CATG^[CT]
PflMI 257 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 695 CACGTG CAC^GTG
PmlI 695 CACGTG CAC^GTG
PvuII 502 CAGCTG CAG^CTG
RcaI 560 TCATGA T^CATGA
Sau3AI 10 GATC ^GATC
ScaI 411 AGTACT AGT^ACT
SfcI 304 CTACAG C^T[GA][CT]AG
SmlI 673 CTTAAG C^T[CT][GA]AG
SphI 200 GCATGC GCATG^C
StuI 54 AGGCCT AGG^CCT
Tru9I 674 TTAA T^TAA
Van91I 257 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 9 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 412 GTAC GT^AC
763 GTAC
AluI 503 AGCT AG^CT
566 AGCT
AlwI 10 GATCC GGATC(4/5)
9 GGATC
BanII 178 GAGCCC G[GA]GC[CT]^C
949 GAGCCC
BbsI 888 GAAGAC GAAGAC(2/6)
490 GTCTTC
BpuAI 888 GAAGAC GAAGAC(2/6)
490 GTCTTC
BsrI 29 CCAGT ACTGG(1/-1)
285 CCAGT
BstXI 245 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
626 CCATTGGCCTGG
CfoI 399 GCGC GCG^C
909 GCGC
DdeI 77 CTCAG C^T[ACGT]AG
161 CTGAG
DsaI 359 CCATGG C^C[GA][CT]GG
782 CCATGG
HhaI 399 GCGC GCG^C
909 GCGC
HinP1I 399 GCGC G^CGC
909 GCGC
HphI 390 TCACC GGTGA(8/7)
431 TCACC
MaeII 418 ACGT A^CGT
696 ACGT
MslI 550 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
736 CATGTGTGTG
MspA1I 502 CAGCTG C[AC]G^C[GT]G
817 CCGCTG
NcoI 359 CCATGG C^CATGG
782 CCATGG
PleI 637 GACTC GAGTC(4/5)
811 GACTC
RsaI 412 GTAC GT^AC
763 GTAC
TaqI 903 TCGA T^CGA
918 TCGA
TthHB8I 903 TCGA T^CGA
918 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AspHI BalI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboII MluNI
MnlI MscI MvaI MwoI NlaIII
NlaIV ScrFI SfaNI StyI TfiI
TseI Tsp45I Tsp509I TspRI XcmI