Restriction map for
pFN21AE3115
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsiYI BslI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MflI
MluI MluNI MroI MscI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 623 GTAC GT^AC
AspHI 3 GAGCTC G[AT]GC[AT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BpmI 531 CTCCAG CTGGAG(16/14)
BpuAI 403 GAAGAC GAAGAC(2/6)
BsiEI 46 CGGTCG CG[GA][CT]^CG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsmAI 757 GAGAC GTCTC(1/5)
BsmBI 757 GAGACG CGTCTC(1/5)
BstXI 830 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 104 GCGAGC GC[ACGT]^[ACGT]GC
DpnII 675 GATC ^GATC
DrdI 73 GACGGTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 604 GAATTC G^AATTC
EcoT14I 584 CCAAGG C^C[AT][AT]GG
FauI 101 CCCGC CCCGC(4/6)
HapII 65 CCGG C^CGG
HpaII 65 CCGG C^CGG
HphI 166 GGTGA GGTGA(8/7)
MboI 675 GATC ^GATC
MfeI 497 CAATTG C^AATTG
MspA1I 427 CAGCTG C[AC]G^C[GT]G
MspI 65 CCGG C^CGG
MunI 497 CAATTG C^AATTG
NdeII 675 GATC ^GATC
PpuMI 97 GGGACCC [GA]G^G[AT]CC[CT]
PstI 647 CTGCAG CTGCA^G
PvuII 427 CAGCTG CAG^CTG
RsaI 623 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
Sau3AI 675 GATC ^GATC
SmaI 64 CCCGGG CCC^GGG
SspI 310 AATATT AAT^ATT
StyI 584 CCAAGG C^C[AT][AT]GG
TaqI 198 TCGA T^CGA
TthHB8I 198 TCGA T^CGA
XcmI 854 CCAGGCCCTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 64 CCCGGG C^CCGGG
XmaI 64 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccII 44 CGCG CG^CG
103 CGCG
AcsI 295 AAATTT [GA]^AATT[CT]
604 GAATTC
ApoI 295 AAATTT [GA]^AATT[CT]
604 GAATTC
AvaII 98 GGACC G^G[AT]CC
581 GGACC
BbvI 649 GCAGC GCAGC(8/12)
31 GCTGC
BcnI 64 CCCGG CC^[GC]GG
65 CCGGG
BstUI 44 CGCG CG^CG
103 CGCG
DraII 97 GGGACCC [GA]G^G[ACGT]CC[CT]
856 AGGCCCT
EcoO109I 97 GGGACCC [GA]G^G[ACGT]CC[CT]
856 AGGCCCT
HaeIII 824 GGCC GG^CC
857 GGCC
MslI 109 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
721 CATCATCATG
MvnI 44 CGCG CG^CG
103 CGCG
NciI 64 CCCGG CC^[GC]GG
65 CCGGG
NlaIV 97 GGGACC GG[ACGT]^[ACGT]CC
98 GGACCC
SfcI 647 CTGCAG C^T[GA][CT]AG
710 CTACAG
SmlI 668 CTCAAG C^T[CT][GA]AG
804 CTCAAG
TseI 31 GCTGC G^C[AT]GC
649 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaI BanII BmyI
BsaJI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI CfoI Cfr13I
EarI EcoRII Fnu4HI FokI HaeII
HhaI HinP1I HinfI ItaI Ksp632I
MboII MnlI MseI MvaI MwoI
NlaIII Sau96I ScrFI SfaNI TfiI
Tru9I Tsp509I TspRI