Restriction map for
pFN21AE3263
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BanII BbeI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 524 GGTACC G^GTACC
AccI 658 GTAGAC GT^[AC][GT]AC
AccII 1526 CGCG CG^CG
Alw44I 411 GTGCAC G^TGCAC
AlwNI 1382 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 411 GTGCAC G^TGCAC
Asp718I 524 GGTACC G^GTACC
AspHI 411 GTGCAC G[AT]GC[AT]^C
BamHI 671 GGATCC G^GATCC
BbvCI 792 GCTGAGG CCTCAGC(-5/-2)
BglII 1197 AGATCT A^GATCT
BsaBI 220 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 955 GAGACC GGTCTC(1/5)
BseRI 1171 GAGGAG GAGGAG(10/8)
BsgI 1078 CTGCAC GTGCAG(16/14)
BsiHKAI 411 GTGCAC G[AT]GC[AT]^C
BsmI 1782 GAATGC GAATGC(1/-1)
BspHI 1337 TCATGA T^CATGA
BspMI 651 GCAGGT ACCTGC(4/8)
BsrI 1247 ACTGG ACTGG(1/-1)
BstUI 1526 CGCG CG^CG
BstXI 1288 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 574 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 966 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 44 CCAAGG C^C[AT][AT]GG
FauI 1662 GCGGG CCCGC(4/6)
HgaI 462 GCGTC GACGC(5/10)
HincII 1585 GTTGAC GT[CT]^[GA]AC
HindII 1585 GTTGAC GT[CT]^[GA]AC
KpnI 524 GGTACC GGTAC^C
MamI 220 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1526 CGCG CG^CG
RcaI 1337 TCATGA T^CATGA
SapI 1369 GAAGAGC GCTCTTC(1/4)
ScaI 1244 AGTACT AGT^ACT
SmlI 1201 CTTGAG C^T[CT][GA]AG
StyI 44 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 525 GTAC GT^AC
1245 GTAC
AflIII 1833 ACACGT A^C[GA][CT]GT
1835 ACGTGT
BanI 33 GGTGCC G^G[CT][GA]CC
524 GGTACC
BbrPI 1721 CACGTG CAC^GTG
1834 CACGTG
BclI 419 TGATCA T^GATCA
906 TGATCA
BcnI 1637 CCCGG CC^[GC]GG
1694 CCCGG
BfaI 805 CTAG C^TAG
1649 CTAG
BmyI 411 GTGCAC G[AGT]GC[ACT]^C
622 GGGCAC
BsaAI 1721 CACGTG [CT]AC^GT[GA]
1834 CACGTG
BsmAI 955 GAGAC GTCTC(1/5)
609 GTCTC
Bsp1286I 411 GTGCAC G[AGT]GC[ACT]^C
622 GGGCAC
FbaI 419 TGATCA T^GATCA
906 TGATCA
HapII 1638 CCGG C^CGG
1695 CCGG
HpaII 1638 CCGG C^CGG
1695 CCGG
HphI 561 GGTGA GGTGA(8/7)
1755 GGTGA
MaeI 805 CTAG C^TAG
1649 CTAG
MaeIII 562 GTGAC ^GT[ACGT]AC
1724 GTGAC
MslI 705 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
736 CACCTCCGTG
MspI 1638 CCGG C^CGG
1695 CCGG
NciI 1637 CCCGG CC^[GC]GG
1694 CCCGG
PflMI 529 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
681 CCACCCTCTGG
PmaCI 1721 CACGTG CAC^GTG
1834 CACGTG
PmlI 1721 CACGTG CAC^GTG
1834 CACGTG
PpuMI 505 AGGACCC [GA]G^G[AT]CC[CT]
1512 GGGACCC
RsaI 525 GTAC GT^AC
1245 GTAC
TfiI 941 GATTC G^A[AT]TC
1800 GAATC
Tsp45I 562 GTGAC ^GT[GC]AC
1724 GTGAC
Van91I 529 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
681 CCACCCTCTGG
XcmI 305 CCAAAAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
719 CCATCACTGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbsI BbvI BpmI BpuAI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SspI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII