Restriction map for
pFN21AE5959
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AlwNI 16 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 400 GTGCTC G[AT]GC[AT]^C
BanI 292 GGCACC G^G[CT][GA]CC
BanII 453 GGGCTC G[GA]GC[CT]^C
BfaI 343 CTAG C^TAG
BglI 346 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 59 AGATCT A^GATCT
BlpI 912 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 912 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
BsmAI 422 GTCTC GTCTC(1/5)
BspHI 404 TCATGA T^CATGA
BstXI 236 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 912 GCTGAGC GC^T[ACGT]AGC
DraII 539 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 350 CCATGG C^C[GA][CT]GG
EarI 748 CTCTTC CTCTTC(1/4)
EcoO109I 539 AGGTCCC [GA]G^G[ACGT]CC[CT]
Ksp632I 748 CTCTTC CTCTTC(1/4)
MaeI 343 CTAG C^TAG
MspA1I 253 CAGCTG C[AC]G^C[GT]G
NcoI 350 CCATGG C^CATGG
NspI 776 ACATGC [GA]CATG^[CT]
PflMI 15 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GACTC GAGTC(4/5)
PpuMI 539 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 253 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SfaNI 433 GCATC GCATC(5/9)
SspI 124 AATATT AAT^ATT
StuI 707 AGGCCT AGG^CCT
Van91I 15 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 144 GATCC GGATC(4/5)
509 GATCC
AvaII 279 GGTCC G^G[AT]CC
540 GGTCC
BalI 8 TGGCCA TGG^CCA
257 TGGCCA
BmyI 400 GTGCTC G[AGT]GC[ACT]^C
453 GGGCTC
BsgI 326 GTGCAG GTGCAG(16/14)
919 GTGCAG
BsmFI 232 GTCCC GGGAC(10/14)
541 GTCCC
Bsp1286I 400 GTGCTC G[AGT]GC[ACT]^C
453 GGGCTC
BspMI 311 ACCTGC ACCTGC(4/8)
718 ACCTGC
BstYI 59 AGATCT [GA]^GATC[CT]
508 AGATCC
Cfr13I 279 GGTCC G^G[ACGT]CC
540 GGTCC
EcoT14I 282 CCTTGG C^C[AT][AT]GG
350 CCATGG
MflI 59 AGATCT [GA]^GATC[CT]
508 AGATCC
MluNI 8 TGGCCA TGG^CCA
257 TGGCCA
MscI 8 TGGCCA TGG^CCA
257 TGGCCA
NlaIV 292 GGCACC GG[ACGT]^[ACGT]CC
540 GGTCCC
PstI 313 CTGCAG CTGCA^G
504 CTGCAG
Sau96I 279 GGTCC G^G[ACGT]CC
540 GGTCC
SfcI 313 CTGCAG C^T[GA][CT]AG
504 CTGCAG
StyI 282 CCTTGG C^C[AT][AT]GG
350 CCATGG
XcmI 11 CCAACCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
15 CCAGACCCTGGGTGG
XhoII 59 AGATCT [GA]^GATC[CT]
508 AGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI TfiI
TseI Tsp45I TspRI