Restriction map for
pFN21AE6022
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KpnI KspI MamI
MluI MroI MspA1I MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 778 GTCTAC GT^[AC][GT]AC
AciI 375 CCGC CCGC(-3/-1)
AcsI 343 GAATTT [GA]^AATT[CT]
AcyI 166 GACGCC G[GA]^CG[CT]C
ApoI 343 GAATTT [GA]^AATT[CT]
AspI 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 261 GGACC G^G[AT]CC
BbsI 838 GTCTTC GAAGAC(2/6)
BglI 358 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 838 GTCTTC GAAGAC(2/6)
BsaAI 379 TACGTG [CT]AC^GT[GA]
BsaHI 166 GACGCC G[GA]^CG[CT]C
BsaWI 858 TCCGGT [AT]^CCGG[AT]
BspHI 416 TCATGA T^CATGA
BsrI 31 ACTGG ACTGG(1/-1)
BstYI 520 AGATCC [GA]^GATC[CT]
DsaI 362 CCATGG C^C[GA][CT]GG
EcoNI 258 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 362 CCATGG C^C[AT][AT]GG
HgaI 166 GACGC GACGC(5/10)
Hin1I 166 GACGCC G[GA]^CG[CT]C
HincII 924 GTTAAC GT[CT]^[GA]AC
HindII 924 GTTAAC GT[CT]^[GA]AC
HpaI 924 GTTAAC GTT^AAC
MaeII 380 ACGT A^CGT
MfeI 205 CAATTG C^AATTG
MflI 520 AGATCC [GA]^GATC[CT]
MunI 205 CAATTG C^AATTG
NcoI 362 CCATGG C^CATGG
NlaIV 458 GGTTCC GG[ACGT]^[ACGT]CC
NspI 788 ACATGC [GA]CATG^[CT]
PflFI 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 264 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 59 GACTC GAGTC(4/5)
PshAI 747 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 516 CTGCAG CTGCA^G
RcaI 416 TCATGA T^CATGA
SexAI 263 ACCAGGT A^CC[AT]GGT
StuI 719 AGGCCT AGG^CCT
StyI 362 CCATGG C^C[AT][AT]GG
Tth111I 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 264 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 248 CCAAGATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 520 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 290 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
519 CAGATCCTG
BanII 465 GGGCTC G[GA]GC[CT]^C
908 GGGCTC
BbvI 452 GCTGC GCAGC(8/12)
772 GCTGC
BfaI 331 CTAG C^TAG
355 CTAG
BsaI 598 GAGACC GGTCTC(1/5)
285 GGTCTC
BsmFI 57 GGGAC GGGAC(10/14)
553 GTCCC
BspMI 730 ACCTGC ACCTGC(4/8)
334 GCAGGT
Cfr13I 261 GGACC G^G[ACGT]CC
708 GGGCC
EarI 61 CTCTTC CTCTTC(1/4)
760 CTCTTC
Fnu4HI 452 GCTGC GC^[ACGT]GC
772 GCTGC
HapII 711 CCGG C^CGG
859 CCGG
HpaII 711 CCGG C^CGG
859 CCGG
ItaI 452 GCTGC GC^[ACGT]GC
772 GCTGC
Ksp632I 61 CTCTTC CTCTTC(1/4)
760 CTCTTC
MaeI 331 CTAG C^TAG
355 CTAG
MseI 874 TTAA T^TAA
925 TTAA
MspI 711 CCGG C^CGG
859 CCGG
MwoI 246 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
358 GCCACCATGGC
Sau96I 261 GGACC G^G[ACGT]CC
708 GGGCC
SfcI 516 CTGCAG C^T[GA][CT]AG
879 CTATAG
TfiI 531 GATTC G^A[AT]TC
935 GATTC
Tru9I 874 TTAA T^TAA
925 TTAA
TseI 452 GCTGC G^C[AT]GC
772 GCTGC
Tsp509I 206 AATT ^AATT
344 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AlwI BalI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI DdeI
DpnII EaeI EcoRII FokI HaeIII
HinfI HphI MaeIII MboI MboII
MluNI MnlI MscI MslI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI Tsp45I TspRI