Restriction map for
pFN21AE6050
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BanI BbeI
BbrPI BbsI BcgI BciVI BcnI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HapII HgaI HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MseI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 586 GACGTC GACGT^C
AccII 213 CGCG CG^CG
AcsI 699 GAATTC [GA]^AATT[CT]
AcyI 586 GACGTC G[GA]^CG[CT]C
ApoI 699 GAATTC [GA]^AATT[CT]
AspHI 3 GTGCTC G[AT]GC[AT]^C
AvaI 798 CCCGAG C^[CT]CG[GA]G
BamHI 308 GGATCC G^GATCC
BbvCI 343 GCTGAGG CCTCAGC(-5/-2)
BclI 749 TGATCA T^GATCA
BfaI 527 CTAG C^TAG
BglI 454 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 817 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC
BsaAI 609 CACGTA [CT]AC^GT[GA]
BsaHI 586 GACGTC G[GA]^CG[CT]C
BsiHKAI 3 GTGCTC G[AT]GC[AT]^C
BsmBI 588 CGTCTC CGTCTC(1/5)
BsmFI 60 GGGAC GGGAC(10/14)
BsmI 138 GAATGC GAATGC(1/-1)
BsoBI 798 CCCGAG C^[CT]CG[GA]G
BspMI 733 ACCTGC ACCTGC(4/8)
BstUI 213 CGCG CG^CG
BstXI 267 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 817 GCTGAGC GC^T[ACGT]AGC
CfoI 214 GCGC GCG^C
DsaI 365 CCATGG C^C[GA][CT]GG
EarI 766 CTCTTC CTCTTC(1/4)
EcoRI 699 GAATTC G^AATTC
FbaI 749 TGATCA T^GATCA
HhaI 214 GCGC GCG^C
Hin1I 586 GACGTC G[GA]^CG[CT]C
HinP1I 214 GCGC G^CGC
Ksp632I 766 CTCTTC CTCTTC(1/4)
MaeI 527 CTAG C^TAG
MvnI 213 CGCG CG^CG
NcoI 365 CCATGG C^CATGG
NlaIV 308 GGATCC GG[ACGT]^[ACGT]CC
PflMI 438 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 815 CAGCTG CAG^CTG
SapI 765 GCTCTTC GCTCTTC(1/4)
SphI 81 GCATGC GCATG^C
StuI 722 AGGCCT AGG^CCT
Tsp509I 700 AATT ^AATT
Van91I 438 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 251 CCAAGATGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 439 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
885 CAGTCTCTG
BalI 711 TGGCCA TGG^CCA
728 TGGCCA
BanII 169 GAGCCC G[GA]GC[CT]^C
911 GGGCTC
BstYI 222 GGATCT [GA]^GATC[CT]
308 GGATCC
Cfr13I 126 GGCCC G^G[ACGT]CC
264 GGGCC
EaeI 711 TGGCCA [CT]^GGCC[GA]
728 TGGCCA
EcoT14I 365 CCATGG C^C[AT][AT]GG
725 CCTTGG
FauI 173 CCCGC CCCGC(4/6)
411 CCCGC
MaeII 587 ACGT A^CGT
610 ACGT
MflI 222 GGATCT [GA]^GATC[CT]
308 GGATCC
MluNI 711 TGGCCA TGG^CCA
728 TGGCCA
MscI 711 TGGCCA TGG^CCA
728 TGGCCA
NspI 81 GCATGC [GA]CATG^[CT]
791 ACATGC
PleI 62 GACTC GAGTC(4/5)
555 GACTC
Sau96I 126 GGCCC G^G[ACGT]CC
264 GGGCC
StyI 365 CCATGG C^C[AT][AT]GG
725 CCTTGG
TfiI 12 GAATC G^A[AT]TC
27 GAATC
XhoII 222 GGATCT [GA]^GATC[CT]
308 GGATCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MvaI MwoI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI TseI Tsp45I TspRI