Restriction map for
pFN21AE6084
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BfrI BglI
BlnI BmyI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 395 GGTACC G^GTACC
AcsI 334 GAATTC [GA]^AATT[CT]
AhdI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 334 GAATTC [GA]^AATT[CT]
Asp718I 395 GGTACC G^GTACC
AspEI 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 252 GGACC G^G[AT]CC
BanI 395 GGTACC G^G[CT][GA]CC
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BciVI 210 GGATAC GTATCC(6/5)
BclI 959 TGATCA T^GATCA
BfaI 916 CTAG C^TAG
BglII 302 AGATCT A^GATCT
BlpI 189 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 189 GCTCAGC GC^T[ACGT]AGC
BsaAI 720 TACGTG [CT]AC^GT[GA]
BseRI 403 CTCCTC GAGGAG(10/8)
BsgI 293 GTGCAG GTGCAG(16/14)
BsmFI 235 GTCCC GGGAC(10/14)
BsrFI 701 GCCGGC [GA]^CCGG[CT]
BstAPI 416 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 101 CCATCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 189 GCTCAGC GC^T[ACGT]AGC
CfoI 705 GCGC GCG^C
Cfr10I 701 GCCGGC [GA]^CCGG[CT]
DraII 251 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 253 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 258 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 251 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 334 GAATTC G^AATTC
FauI 161 CCCGC CCCGC(4/6)
FbaI 959 TGATCA T^GATCA
HhaI 705 GCGC GCG^C
HinP1I 705 GCGC G^CGC
KpnI 395 GGTACC GGTAC^C
MaeI 916 CTAG C^TAG
NaeI 701 GCCGGC GCC^GGC
NgoMIV 701 GCCGGC G^CCGGC
PflMI 732 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 251 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 480 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 896 CTGCAG CTGCA^G
ScaI 912 AGTACT AGT^ACT
SmaI 73 CCCGGG CCC^GGG
SmlI 876 CTTGAG C^T[CT][GA]AG
StuI 48 AGGCCT AGG^CCT
TfiI 138 GATTC G^A[AT]TC
Tsp509I 335 AATT ^AATT
TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 732 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 575 ACACGT A^C[GA][CT]GT
721 ACGTGT
AlwI 512 GATCC GGATC(4/5)
543 GATCC
AvaI 73 CCCGGG C^[CT]CG[GA]G
515 CCCGAG
BbsI 14 GTCTTC GAAGAC(2/6)
94 GTCTTC
BcnI 73 CCCGG CC^[GC]GG
74 CCGGG
BpuAI 14 GTCTTC GAAGAC(2/6)
94 GTCTTC
BsaI 589 GAGACC GGTCTC(1/5)
349 GGTCTC
BsoBI 73 CCCGGG C^[CT]CG[GA]G
515 CCCGAG
BspHI 134 TCATGA T^CATGA
407 TCATGA
Cfr13I 252 GGACC G^G[ACGT]CC
699 GGGCC
EcoT14I 263 CCAAGG C^C[AT][AT]GG
461 CCTTGG
HapII 74 CCGG C^CGG
702 CCGG
HpaII 74 CCGG C^CGG
702 CCGG
MaeIII 487 GTCAC ^GT[ACGT]AC
579 GTCAC
MspI 74 CCGG C^CGG
702 CCGG
NciI 73 CCCGG CC^[GC]GG
74 CCGGG
PleI 157 GACTC GAGTC(4/5)
480 GACTC
RcaI 134 TCATGA T^CATGA
407 TCATGA
Sau96I 252 GGACC G^G[ACGT]CC
699 GGGCC
StyI 263 CCAAGG C^C[AT][AT]GG
461 CCTTGG
Tsp45I 487 GTCAC ^GT[GC]AC
579 GTCAC
XcmI 101 CCATCTTTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
275 CCATTTCCTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MboI
MboII MflI MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI SfcI TseI
XhoII