Restriction map for
pFN21AE5390
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MfeI MflI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 331 GAATTC [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
ApoI 331 GAATTC [GA]^AATT[CT]
AvaII 269 GGACC G^G[AT]CC
BsaBI 660 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 288 CTGCAC GTGCAG(16/14)
BsmAI 494 GTCTC GTCTC(1/5)
BspHI 131 TCATGA T^CATGA
BspMI 718 ACCTGC ACCTGC(4/8)
BsrDI 613 GCAATG GCAATG(2/0)
BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 390 GCGC GCG^C
Cfr13I 269 GGACC G^G[ACGT]CC
EcoNI 650 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 331 GAATTC G^AATTC
FauI 414 GCGGG CCCGC(4/6)
HhaI 390 GCGC GCG^C
HinP1I 390 GCGC G^CGC
MamI 660 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 408 CAGCTG C[AC]G^C[GT]G
PflMI 437 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 492 GAGTC GAGTC(4/5)
PvuII 408 CAGCTG CAG^CTG
RcaI 131 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
Sau96I 269 GGACC G^G[ACGT]CC
SfcI 867 CTACAG C^T[GA][CT]AG
SnaBI 771 TACGTA TAC^GTA
StuI 707 AGGCCT AGG^CCT
Tsp509I 332 AATT ^AATT
Van91I 437 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 548 CCATGCTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 768 GTATAC GT^[AC][GT]AC
774 GTATAC
AciI 363 CCGC CCGC(-3/-1)
414 GCGG
AlwI 429 GATCC GGATC(4/5)
624 GATCC
BanI 540 GGCACC G^G[CT][GA]CC
895 GGTGCC
BanII 46 GGGCTC G[GA]GC[CT]^C
346 GGGCTC
BbsI 100 GTCTTC GAAGAC(2/6)
817 GTCTTC
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
552 GCTGAGG
BpuAI 100 GTCTTC GAAGAC(2/6)
817 GTCTTC
BsaAI 367 TACGTG [CT]AC^GT[GA]
771 TACGTA
BseRI 184 CTCCTC GAGGAG(10/8)
472 CTCCTC
BsmFI 268 GGGAC GGGAC(10/14)
569 GGGAC
BssKI 432 CCTGG ^CC[ACGT]GG
656 CCAGG
Bst1107I 768 GTATAC GTA^TAC
774 GTATAC
BstNI 432 CCTGG CC^[AT]GG
656 CCAGG
BstZ17I 768 GTATAC GTA^TAC
774 GTATAC
EcoRII 432 CCTGG ^CC[AT]GG
656 CCAGG
MaeII 368 ACGT A^CGT
772 ACGT
MaeIII 640 GTGAC ^GT[ACGT]AC
742 GTGAC
MseI 146 TTAA T^TAA
853 TTAA
MvaI 432 CCTGG CC^[AT]GG
656 CCAGG
SapI 48 GCTCTTC GCTCTTC(1/4)
722 GCTCTTC
ScrFI 432 CCTGG CC^[ACGT]GG
656 CCAGG
TfiI 74 GATTC G^A[AT]TC
660 GATTC
Tru9I 146 TTAA T^TAA
853 TTAA
Tsp45I 640 GTGAC ^GT[GC]AC
742 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BalI BbvI BmyI BsiYI
BslI Bsp1286I BstF5I Cac8I DdeI
DpnII EaeI EarI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MboI MboII MluNI MnlI MscI
MslI MwoI NdeII NlaIII NlaIV
Sau3AI SfaNI TseI TspRI