Restriction map for
pFN21AE6060
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AlwNI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI BspEI BsrBI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I CelII CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MroI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspI SwaI Tsp45I Tsp509I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 274 CGCG CG^CG
Alw44I 503 GTGCAC G^TGCAC
AocI 417 CCTGAGG CC^T[ACGT]AGG
ApaLI 503 GTGCAC G^TGCAC
AspHI 503 GTGCAC G[AT]GC[AT]^C
AspI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 36 CCTAGG C^CTAGG
BalI 89 TGGCCA TGG^CCA
BanII 59 GAGCCC G[GA]GC[CT]^C
BbsI 574 GTCTTC GAAGAC(2/6)
BfaI 37 CTAG C^TAG
BglII 293 AGATCT A^GATCT
BlnI 36 CCTAGG C^CTAGG
BpuAI 574 GTCTTC GAAGAC(2/6)
BsaWI 253 ACCGGA [AT]^CCGG[AT]
BsiHKAI 503 GTGCAC G[AT]GC[AT]^C
Bsp1407I 590 TGTACA T^GTACA
BspDI 514 ATCGAT AT^CGAT
BspLU11I 723 ACATGT A^CATGT
BspMI 712 ACCTGC ACCTGC(4/8)
BsrDI 116 GCAATG GCAATG(2/0)
BsrFI 152 ACCGGC [GA]^CCGG[CT]
BsrGI 590 TGTACA T^GTACA
BssSI 508 CACGAG CACGAG(-5/-1)
BstBI 577 TTCGAA TT^CGAA
BstUI 274 CGCG CG^CG
BstYI 293 AGATCT [GA]^GATC[CT]
Bsu36I 417 CCTGAGG CC^T[ACGT]AGG
Cfr10I 152 ACCGGC [GA]^CCGG[CT]
ClaI 514 ATCGAT AT^CGAT
DraII 533 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 311 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 344 CCATGG C^C[GA][CT]GG
EaeI 89 TGGCCA [CT]^GGCC[GA]
Eco81I 417 CCTGAGG CC^T[ACGT]AGG
EcoNI 462 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 533 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 386 GATATC GAT^ATC
HphI 836 TCACC GGTGA(8/7)
MaeI 37 CTAG C^TAG
MflI 293 AGATCT [GA]^GATC[CT]
MluNI 89 TGGCCA TGG^CCA
MscI 89 TGGCCA TGG^CCA
MvnI 274 CGCG CG^CG
NcoI 344 CCATGG C^CATGG
NspI 723 ACATGT [GA]CATG^[CT]
NspV 577 TTCGAA TT^CGAA
PflFI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 466 CTGCAG CTGCA^G
SapI 716 GCTCTTC GCTCTTC(1/4)
SbfI 465 CCTGCAGG CCTGCA^GG
SfuI 577 TTCGAA TT^CGAA
Sse8387I 465 CCTGCAGG CCTGCA^GG
SspBI 590 TGTACA T^GTACA
StuI 701 AGGCCT AGG^CCT
Tth111I 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 293 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 171 GTAC GT^AC
591 GTAC
AflIII 723 ACATGT A^C[GA][CT]GT
913 ACGTGT
BsaI 774 GAGACC GGTCTC(1/5)
649 GGTCTC
BsgI 289 GTGCAG GTGCAG(16/14)
449 GTGCAG
BsmAI 774 GAGAC GTCTC(1/5)
650 GTCTC
BsrI 143 ACTGG ACTGG(1/-1)
312 ACTGG
CfoI 275 GCGC GCG^C
678 GCGC
DrdI 426 GACCATGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
565 GACACTTCAGTC
EarI 73 CTCTTC CTCTTC(1/4)
717 CTCTTC
EcoT14I 36 CCTAGG C^C[AT][AT]GG
344 CCATGG
FauI 272 CCCGC CCCGC(4/6)
692 CCCGC
HapII 153 CCGG C^CGG
254 CCGG
HhaI 275 GCGC GCG^C
678 GCGC
HinP1I 275 GCGC G^CGC
678 GCGC
HpaII 153 CCGG C^CGG
254 CCGG
Ksp632I 73 CTCTTC CTCTTC(1/4)
717 CTCTTC
MaeII 203 ACGT A^CGT
913 ACGT
MseI 47 TTAA T^TAA
607 TTAA
MspA1I 409 CAGCTG C[AC]G^C[GT]G
452 CAGCTG
MspI 153 CCGG C^CGG
254 CCGG
MwoI 156 GCTCCACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
340 GCAGCCATGGC
PleI 41 GACTC GAGTC(4/5)
740 GACTC
PvuII 409 CAGCTG CAG^CTG
452 CAGCTG
RsaI 171 GTAC GT^AC
591 GTAC
SfaNI 81 GATGC GCATC(5/9)
207 GATGC
StyI 36 CCTAGG C^C[AT][AT]GG
344 CCATGG
TaqI 515 TCGA T^CGA
578 TCGA
TfiI 129 GATTC G^A[AT]TC
138 GATTC
Tru9I 47 TTAA T^TAA
607 TTAA
TthHB8I 515 TCGA T^CGA
578 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspHI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MboII MnlI MslI MvaI NdeII
NlaIII NlaIV RcaI Sau3AI Sau96I
ScrFI SfcI TseI TspRI