Restriction map for
pFN21AE5977
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tru9I Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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BalI 95 TGGCCA TGG^CCA
BamHI 446 GGATCC G^GATCC
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BglII 908 AGATCT A^GATCT
BsaI 509 GGTCTC GGTCTC(1/5)
BsgI 222 CTGCAC GTGCAG(16/14)
BsmAI 510 GTCTC GTCTC(1/5)
BsmFI 198 GTCCC GGGAC(10/14)
Bsp1407I 773 TGTACA T^GTACA
BsrDI 642 CATTGC GCAATG(2/0)
BsrGI 773 TGTACA T^GTACA
BstEII 888 GGTGACC G^GT[ACGT]ACC
BstPI 888 GGTGACC G^GT[ACGT]ACC
BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 95 TGGCCA [CT]^GGCC[GA]
EarI 79 CTCTTC CTCTTC(1/4)
EcoO65I 888 GGTGACC G^GT[ACGT]ACC
EcoRI 12 GAATTC G^AATTC
HindIII 707 AAGCTT A^AGCTT
Ksp632I 79 CTCTTC CTCTTC(1/4)
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
NlaIV 446 GGATCC GG[ACGT]^[ACGT]CC
PaeR7I 656 CTCGAG C^TCGAG
SfcI 867 CTACAG C^T[GA][CT]AG
SmlI 656 CTCGAG C^T[CT][GA]AG
SspBI 773 TGTACA T^GTACA
SspI 579 AATATT AAT^ATT
XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 656 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
876 CCGC
Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
586 GAAAAGATTC
AvaI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
AvaII 90 GGTCC G^G[AT]CC
207 GGACC
BanII 408 GAGCCC G[GA]GC[CT]^C
453 GGGCTC
BbvI 263 GCAGC GCAGC(8/12)
760 GCAGC
BmyI 408 GAGCCC G[AGT]GC[ACT]^C
453 GGGCTC
BpmI 689 CTGGAG CTGGAG(16/14)
929 CTGGAG
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
450 CCTGGG
BsmI 331 GAATGC GAATGC(1/-1)
898 GCATTC
BsoBI 512 CTCGGG C^[CT]CG[GA]G
656 CTCGAG
Bsp1286I 408 GAGCCC G[AGT]GC[ACT]^C
453 GGGCTC
BspHI 404 TCATGA T^CATGA
902 TCATGA
Fnu4HI 263 GCAGC GC^[ACGT]GC
760 GCAGC
HphI 888 GGTGA GGTGA(8/7)
729 TCACC
ItaI 263 GCAGC GC^[ACGT]GC
760 GCAGC
RcaI 404 TCATGA T^CATGA
902 TCATGA
TaqI 16 TCGA T^CGA
657 TCGA
TseI 263 GCAGC G^C[AT]GC
760 GCAGC
TthHB8I 16 TCGA T^CGA
657 TCGA
XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
586 GAAAAGATTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BseRI BsiYI BslI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI MaeIII
MboI MboII MflI MnlI MslI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI TfiI Tsp45I
Tsp509I TspRI XhoII