Restriction map for
pFN21AE5970
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MroI
MseI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 670 GTCTAC GT^[AC][GT]AC
AciI 675 CCGC CCGC(-3/-1)
AfaI 775 GTAC GT^AC
ApaI 893 GGGCCC GGGCC^C
Asp700I 915 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 368 TGGCCA TGG^CCA
BanII 893 GGGCCC G[GA]GC[CT]^C
BciVI 491 GTATCC GTATCC(6/5)
BcnI 654 CCGGG CC^[GC]GG
BfaI 556 CTAG C^TAG
BsgI 561 CTGCAC GTGCAG(16/14)
BssKI 654 CCGGG ^CC[ACGT]GG
BstAPI 754 GCACCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 568 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HapII 654 CCGG C^CGG
HgaI 552 GACGC GACGC(5/10)
HincII 549 GTTGAC GT[CT]^[GA]AC
HindII 549 GTTGAC GT[CT]^[GA]AC
HpaII 654 CCGG C^CGG
MaeI 556 CTAG C^TAG
MaeII 777 ACGT A^CGT
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MspI 654 CCGG C^CGG
MwoI 754 GCACCCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 654 CCGGG CC^[GC]GG
NheI 555 GCTAGC G^CTAGC
PstI 686 CTGCAG CTGCA^G
RsaI 775 GTAC GT^AC
ScrFI 654 CCGGG CC^[ACGT]GG
SphI 677 GCATGC GCATG^C
StuI 704 AGGCCT AGG^CCT
XmnI 915 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 785 GATCC GGATC(4/5)
436 GGATC
AspHI 467 GTGCTC G[AT]GC[AT]^C
585 GAGCAC
BbvI 602 GCAGC GCAGC(8/12)
333 GCTGC
BsiHKAI 467 GTGCTC G[AT]GC[AT]^C
585 GAGCAC
BsiYI 625 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
647 CCTATGGCCGG
BslI 625 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
647 CCTATGGCCGG
BsrI 280 ACTGG ACTGG(1/-1)
631 ACTGG
BstYI 436 GGATCT [GA]^GATC[CT]
784 AGATCC
Cfr13I 893 GGGCC G^G[ACGT]CC
894 GGCCC
DraII 892 GGGGCCC [GA]G^G[ACGT]CC[CT]
893 GGGCCCT
EaeI 368 TGGCCA [CT]^GGCC[GA]
651 TGGCCG
EcoO109I 892 GGGGCCC [GA]G^G[ACGT]CC[CT]
893 GGGCCCT
Fnu4HI 333 GCTGC GC^[ACGT]GC
602 GCAGC
ItaI 333 GCTGC GC^[ACGT]GC
602 GCAGC
MflI 436 GGATCT [GA]^GATC[CT]
784 AGATCC
NlaIV 892 GGGGCC GG[ACGT]^[ACGT]CC
893 GGGCCC
NspI 640 GCATGT [GA]CATG^[CT]
677 GCATGC
Sau96I 893 GGGCC G^G[ACGT]CC
894 GGCCC
TseI 333 GCTGC G^C[AT]GC
602 GCAGC
XhoII 436 GGATCT [GA]^GATC[CT]
784 AGATCC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BmyI Bsp1286I BstF5I Cac8I
DdeI DpnII FokI HaeIII HinfI
HphI MboI MboII MnlI MslI
NdeII NlaIII Sau3AI SfcI TfiI
Tsp509I TspRI