Restriction map for
pFN21AE3186
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 17 CGCG CG^CG
AfaI 703 GTAC GT^AC
AseI 160 ATTAAT AT^TAAT
AsnI 160 ATTAAT AT^TAAT
Asp700I 346 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 977 GAGCAC G[AT]GC[AT]^C
AspI 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 137 CCCGGG C^[CT]CG[GA]G
BclI 218 TGATCA T^GATCA
BfaI 586 CTAG C^TAG
BsaBI 660 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 977 GAGCAC G[AT]GC[AT]^C
BsoBI 137 CCCGGG C^[CT]CG[GA]G
BspHI 258 TCATGA T^CATGA
BspMI 820 GCAGGT ACCTGC(4/8)
BsrBI 80 CCGCTC CCGCTC(-3/-3)
BstUI 17 CGCG CG^CG
CfoI 11 GCGC GCG^C
DraII 1144 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1235 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1144 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 939 GATATC GAT^ATC
FbaI 218 TGATCA T^GATCA
HaeII 10 AGCGCC [GA]GCGC^[CT]
HaeIII 1071 GGCC GG^CC
HhaI 11 GCGC GCG^C
HinP1I 11 GCGC G^CGC
MaeI 586 CTAG C^TAG
MamI 660 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1066 CAGCGG C[AC]G^C[GT]G
MvnI 17 CGCG CG^CG
PflFI 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1131 GACTC GAGTC(4/5)
PpuMI 1144 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 160 ATTAAT AT^TAAT
RcaI 258 TCATGA T^CATGA
RsaI 703 GTAC GT^AC
SfaNI 665 GCATC GCATC(5/9)
SfcI 33 CTATAG C^T[GA][CT]AG
SmaI 137 CCCGGG CCC^GGG
SspI 513 AATATT AAT^ATT
TaqI 331 TCGA T^CGA
TfiI 660 GATTC G^A[AT]TC
Tth111I 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 331 TCGA T^CGA
XmaCI 137 CCCGGG C^CCGGG
XmaI 137 CCCGGG C^CCGGG
XmnI 346 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 542 GAATTC [GA]^AATT[CT]
1015 GAATTC
AcyI 763 GACGCC G[GA]^CG[CT]C
1230 GACGCC
AlwNI 417 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGGAGCTG
ApoI 542 GAATTC [GA]^AATT[CT]
1015 GAATTC
AvaII 275 GGACC G^G[AT]CC
1145 GGACC
BanI 51 GGCACC G^G[CT][GA]CC
589 GGCACC
BbsI 177 GAAGAC GAAGAC(2/6)
1200 GAAGAC
BmyI 755 GTGCCC G[AGT]GC[ACT]^C
977 GAGCAC
BpmI 569 CTGGAG CTGGAG(16/14)
750 CTGGAG
BpuAI 177 GAAGAC GAAGAC(2/6)
1200 GAAGAC
BsaHI 763 GACGCC G[GA]^CG[CT]C
1230 GACGCC
BsaI 24 GAGACC GGTCTC(1/5)
183 GGTCTC
BsmAI 24 GAGAC GTCTC(1/5)
184 GTCTC
Bsp1286I 755 GTGCCC G[AGT]GC[ACT]^C
977 GAGCAC
BstAPI 370 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
979 GCACACGCTGC
BstF5I 342 GGATG GGATG(2/0)
1179 GGATG
EarI 958 GAAGAG CTCTTC(1/4)
1249 GAAGAG
EcoRI 542 GAATTC G^AATTC
1015 GAATTC
FauI 18 GCGGG CCCGC(4/6)
1068 GCGGG
FokI 342 GGATG GGATG(9/13)
1179 GGATG
HapII 29 CCGG C^CGG
138 CCGG
HgaI 763 GACGC GACGC(5/10)
1230 GACGC
Hin1I 763 GACGCC G[GA]^CG[CT]C
1230 GACGCC
HinfI 660 GATTC G^A[ACGT]TC
1131 GACTC
HpaII 29 CCGG C^CGG
138 CCGG
Ksp632I 958 GAAGAG CTCTTC(1/4)
1249 GAAGAG
MseI 161 TTAA T^TAA
635 TTAA
MslI 279 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
316 CATTCCTGTG
MspI 29 CCGG C^CGG
138 CCGG
NlaIII 259 CATG CATG^
915 CATG
Tru9I 161 TTAA T^TAA
635 TTAA
XcmI 188 CCACGATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
767 CCATTTATTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI BamHI BbvI
BcnI BsaJI BsiYI BslI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I Tsp509I TspRI XhoII