Restriction map for
pFN21AE2726
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT14I EcoT22I FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 691 GTAC GT^AC
AseI 148 ATTAAT AT^TAAT
AsnI 148 ATTAAT AT^TAAT
Asp700I 334 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 965 GAGCAC G[AT]GC[AT]^C
AspI 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
BclI 206 TGATCA T^GATCA
BfaI 574 CTAG C^TAG
BsaBI 648 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 171 GGTCTC GGTCTC(1/5)
BsiHKAI 965 GAGCAC G[AT]GC[AT]^C
BsmAI 172 GTCTC GTCTC(1/5)
BsoBI 125 CCCGGG C^[CT]CG[GA]G
BspHI 246 TCATGA T^CATGA
BspMI 808 GCAGGT ACCTGC(4/8)
BsrBI 68 CCGCTC CCGCTC(-3/-3)
BssSI 11 CACGAG CACGAG(-5/-1)
DraII 1132 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1223 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1132 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 927 GATATC GAT^ATC
FauI 1056 GCGGG CCCGC(4/6)
FbaI 206 TGATCA T^GATCA
HaeIII 1059 GGCC GG^CC
HapII 126 CCGG C^CGG
HpaII 126 CCGG C^CGG
MaeI 574 CTAG C^TAG
MamI 648 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1054 CAGCGG C[AC]G^C[GT]G
MspI 126 CCGG C^CGG
PflFI 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1119 GACTC GAGTC(4/5)
PpuMI 1132 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 148 ATTAAT AT^TAAT
RcaI 246 TCATGA T^CATGA
RsaI 691 GTAC GT^AC
SfaNI 653 GCATC GCATC(5/9)
SmaI 125 CCCGGG CCC^GGG
SspI 501 AATATT AAT^ATT
TaqI 319 TCGA T^CGA
TfiI 648 GATTC G^A[AT]TC
Tth111I 1134 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 319 TCGA T^CGA
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 334 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 530 GAATTC [GA]^AATT[CT]
1003 GAATTC
AcyI 751 GACGCC G[GA]^CG[CT]C
1218 GACGCC
AlwNI 405 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1111 CAGGAGCTG
ApoI 530 GAATTC [GA]^AATT[CT]
1003 GAATTC
AvaII 263 GGACC G^G[AT]CC
1133 GGACC
BanI 39 GGCACC G^G[CT][GA]CC
577 GGCACC
BbsI 165 GAAGAC GAAGAC(2/6)
1188 GAAGAC
BcnI 125 CCCGG CC^[GC]GG
126 CCGGG
BmyI 743 GTGCCC G[AGT]GC[ACT]^C
965 GAGCAC
BpmI 557 CTGGAG CTGGAG(16/14)
738 CTGGAG
BpuAI 165 GAAGAC GAAGAC(2/6)
1188 GAAGAC
BsaHI 751 GACGCC G[GA]^CG[CT]C
1218 GACGCC
Bsp1286I 743 GTGCCC G[AGT]GC[ACT]^C
965 GAGCAC
BstAPI 358 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
967 GCACACGCTGC
BstF5I 330 GGATG GGATG(2/0)
1167 GGATG
EarI 946 GAAGAG CTCTTC(1/4)
1237 GAAGAG
EcoRI 530 GAATTC G^AATTC
1003 GAATTC
FokI 330 GGATG GGATG(9/13)
1167 GGATG
HgaI 751 GACGC GACGC(5/10)
1218 GACGC
Hin1I 751 GACGCC G[GA]^CG[CT]C
1218 GACGCC
HinfI 648 GATTC G^A[ACGT]TC
1119 GACTC
Ksp632I 946 GAAGAG CTCTTC(1/4)
1237 GAAGAG
MseI 149 TTAA T^TAA
623 TTAA
MslI 267 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
304 CATTCCTGTG
NciI 125 CCCGG CC^[GC]GG
126 CCGGG
NlaIII 247 CATG CATG^
903 CATG
Tru9I 149 TTAA T^TAA
623 TTAA
XcmI 176 CCACGATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
755 CCATTTATTTTGTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BamHI BbvI
BsaJI BsiYI BslI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I
Tsp509I TspRI XhoII