Restriction map for
pFN21AE6096
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbvI BcgI BciVI
BcnI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FauI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KspI MaeI MaeII MfeI
MluI MroI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TfiI Tru9I
TseI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 673 GGTACC G^GTACC
AciI 411 CCGC CCGC(-3/-1)
AflIII 111 ACATGT A^C[GA][CT]GT
Alw44I 239 GTGCAC G^TGCAC
ApaLI 239 GTGCAC G^TGCAC
Asp718I 673 GGTACC G^GTACC
AspHI 239 GTGCAC G[AT]GC[AT]^C
AvaII 206 GGTCC G^G[AT]CC
BamHI 728 GGATCC G^GATCC
BanI 673 GGTACC G^G[CT][GA]CC
BclI 176 TGATCA T^GATCA
BglII 297 AGATCT A^GATCT
BmyI 239 GTGCAC G[AGT]GC[ACT]^C
BsaBI 51 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 256 GAGACC GGTCTC(1/5)
BsgI 527 CTGCAC GTGCAG(16/14)
BsiHKAI 239 GTGCAC G[AT]GC[AT]^C
Bsp1286I 239 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 114 TGTACA T^GTACA
BspLU11I 111 ACATGT A^CATGT
BsrGI 114 TGTACA T^GTACA
BsrI 303 ACTGG ACTGG(1/-1)
BssKI 417 CCTGG ^CC[ACGT]GG
BstF5I 690 CATCC GGATG(2/0)
BstNI 417 CCTGG CC^[AT]GG
BstXI 284 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 206 GGTCC G^G[ACGT]CC
DraII 205 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 785 CCGTGG C^C[GA][CT]GG
EarI 229 CTCTTC CTCTTC(1/4)
EcoO109I 205 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 417 CCTGG ^CC[AT]GG
EcoRV 440 GATATC GAT^ATC
EcoT14I 308 CCAAGG C^C[AT][AT]GG
FbaI 176 TGATCA T^GATCA
FokI 690 CATCC GGATG(9/13)
HinfI 544 GACTC G^A[ACGT]TC
KpnI 673 GGTACC GGTAC^C
Ksp632I 229 CTCTTC CTCTTC(1/4)
MamI 51 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 737 CAGCTG C[AC]G^C[GT]G
MvaI 417 CCTGG CC^[AT]GG
NdeI 586 CATATG CA^TATG
NspI 111 ACATGT [GA]CATG^[CT]
PflMI 585 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 544 GACTC GAGTC(4/5)
PpuMI 205 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 737 CAGCTG CAG^CTG
Sau96I 206 GGTCC G^G[ACGT]CC
ScrFI 417 CCTGG CC^[ACGT]GG
SspBI 114 TGTACA T^GTACA
StuI 755 AGGCCT AGG^CCT
StyI 308 CCAAGG C^C[AT][AT]GG
Tsp45I 328 GTCAC ^GT[GC]AC
Van91I 585 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 340 AGCT AG^CT
738 AGCT
AlwI 729 GATCC GGATC(4/5)
728 GGATC
AlwNI 521 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
656 CAGTGTCTG
BalI 305 TGGCCA TGG^CCA
419 TGGCCA
BbsI 136 GTCTTC GAAGAC(2/6)
703 GTCTTC
BbvCI 891 CCTCAGC CCTCAGC(-5/-2)
739 GCTGAGG
BpuAI 136 GTCTTC GAAGAC(2/6)
703 GTCTTC
BsaJI 308 CCAAGG C^C[ACGT][ACGT]GG
785 CCGTGG
BspMI 259 ACCTGC ACCTGC(4/8)
166 GCAGGT
BstYI 297 AGATCT [GA]^GATC[CT]
728 GGATCC
EaeI 305 TGGCCA [CT]^GGCC[GA]
419 TGGCCA
EcoRI 79 GAATTC G^AATTC
634 GAATTC
MflI 297 AGATCT [GA]^GATC[CT]
728 GGATCC
MluNI 305 TGGCCA TGG^CCA
419 TGGCCA
MscI 305 TGGCCA TGG^CCA
419 TGGCCA
MslI 216 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
393 CACTTTGATG
MwoI 490 GCTTGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
494 GCACTGCAGGC
PshAI 273 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
696 GACTGATGTC
PstI 497 CTGCAG CTGCA^G
552 CTGCAG
SfaNI 288 GATGC GCATC(5/9)
811 GCATC
SfcI 497 CTGCAG C^T[GA][CT]AG
552 CTGCAG
XhoII 297 AGATCT [GA]^GATC[CT]
728 GGATCC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AocI ApoI Asp700I
BsiYI BslI BsmAI BsmFI Bsu36I
Cac8I DdeI DpnII Eco81I HaeIII
HphI MaeIII MboI MboII MnlI
NdeII NlaIII NlaIV RsaI Sau3AI
Tsp509I TspRI XmnI