Restriction map for
pFN21AE5948
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HgaI Hin1I
HinfI HpaI HpaII KasI KpnI
KspI MaeII MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 6 GCGG CCGC(-3/-1)
AflII 777 CTTAAG C^TTAAG
AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 70 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 158 GGGCCC GGGCC^C
Asp700I 920 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 615 GGTCC G^G[AT]CC
BalI 59 TGGCCA TGG^CCA
BanI 118 GGCACC G^G[CT][GA]CC
BanII 158 GGGCCC G[GA]GC[CT]^C
BbsI 267 GAAGAC GAAGAC(2/6)
BciVI 826 GTATCC GTATCC(6/5)
BfaI 430 CTAG C^TAG
BfrI 777 CTTAAG C^TTAAG
BpuAI 267 GAAGAC GAAGAC(2/6)
BsaBI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 16 GAGAC GTCTC(1/5)
Bsp1407I 607 TGTACA T^GTACA
BsrBI 4 GAGCGG CCGCTC(-3/-3)
BsrDI 540 CATTGC GCAATG(2/0)
BsrGI 607 TGTACA T^GTACA
BsrI 693 ACTGG ACTGG(1/-1)
BstAPI 69 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 390 GCGC GCG^C
EaeI 59 TGGCCA [CT]^GGCC[GA]
Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 313 CTCTTC CTCTTC(1/4)
FauI 6 GCGGG CCCGC(4/6)
HhaI 390 GCGC GCG^C
HinP1I 390 GCGC G^CGC
HincII 247 GTTGAC GT[CT]^[GA]AC
HindII 247 GTTGAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
Ksp632I 313 CTCTTC CTCTTC(1/4)
MaeI 430 CTAG C^TAG
MamI 588 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 59 TGGCCA TGG^CCA
MscI 59 TGGCCA TGG^CCA
MseI 778 TTAA T^TAA
MspA1I 73 CAGCTG C[AC]G^C[GT]G
PstI 67 CTGCAG CTGCA^G
PvuII 73 CAGCTG CAG^CTG
SfcI 67 CTGCAG C^T[GA][CT]AG
SpeI 429 ACTAGT A^CTAGT
SspBI 607 TGTACA T^GTACA
StuI 781 AGGCCT AGG^CCT
Tru9I 778 TTAA T^TAA
XmnI 920 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BmyI 117 GGGCAC G[AGT]GC[ACT]^C
158 GGGCCC
BseRI 97 CTCCTC GAGGAG(10/8)
798 CTCCTC
Bsp1286I 117 GGGCAC G[AGT]GC[ACT]^C
158 GGGCCC
BspMI 251 ACCTGC ACCTGC(4/8)
494 ACCTGC
BstXI 143 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCAAGATGCTGG
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
558 GCTGGC
DraII 157 AGGGCCC [GA]G^G[ACGT]CC[CT]
158 GGGCCCT
EcoO109I 157 AGGGCCC [GA]G^G[ACGT]CC[CT]
158 GGGCCCT
EcoT14I 318 CCTTGG C^C[AT][AT]GG
482 CCTTGG
MslI 294 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
594 CATCTTCATG
NlaIV 118 GGCACC GG[ACGT]^[ACGT]CC
158 GGGCCC
StyI 318 CCTTGG C^C[AT][AT]GG
482 CCTTGG
Tsp45I 536 GTGAC ^GT[GC]AC
742 GTCAC
Tsp509I 909 AATT ^AATT
924 AATT
XcmI 140 CCACCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
272 CCATTTCTTTCCTGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
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AfaI AluI BbvI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TseI TspRI