Restriction map for
pFN21AE2436
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbrPI BbsI BcgI BclI
BfaI BfrI BglII BlnI BpuAI
BseAI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MfeI MflI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 2189 AAATTC [GA]^AATT[CT]
AflIII 659 ACGTGT A^C[GA][CT]GT
AgeI 434 ACCGGT A^CCGGT
AhdI 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1932 GTGCAC G^TGCAC
AlwI 3 GATCC GGATC(4/5)
ApaLI 1932 GTGCAC G^TGCAC
ApoI 2189 AAATTC [GA]^AATT[CT]
AspEI 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1529 GGCGCC GGCGC^C
BbvCI 1800 CCTCAGC CCTCAGC(-5/-2)
BciVI 733 GGATAC GTATCC(6/5)
BlpI 2037 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2037 GCTGAGC GC^T[ACGT]AGC
BsaBI 1960 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 434 ACCGGT [AT]^CCGG[AT]
BsiWI 317 CGTACG C^GTACG
BssHII 1503 GCGCGC G^CGCGC
BssSI 2140 CTCGTG CACGAG(-5/-1)
BstAPI 1571 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 822 GGTCACC G^GT[ACGT]ACC
BstPI 822 GGTCACC G^GT[ACGT]ACC
BstXI 1239 CCAGGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2037 GCTGAGC GC^T[ACGT]AGC
DpnII 3 GATC ^GATC
DrdI 110 GACACCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1428 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 948 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 822 GGTCACC G^GT[ACGT]ACC
HincII 1582 GTCAAC GT[CT]^[GA]AC
HindII 1582 GTCAAC GT[CT]^[GA]AC
KasI 1529 GGCGCC G^GCGCC
MamI 1960 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 3 GATC ^GATC
MslI 2016 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1529 GGCGCC GG^CGCC
NdeII 3 GATC ^GATC
NotI 879 GCGGCCGC GC^GGCCGC
PflFI 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1587 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 434 ACCGGT A^CCGGT
PshAI 849 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 3 GATC ^GATC
ScaI 2048 AGTACT AGT^ACT
SfiI 872 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1529 GGCGCC GGC^GCC
SmaI 1214 CCCGGG CCC^GGG
StuI 687 AGGCCT AGG^CCT
Tsp509I 2190 AATT ^AATT
Tth111I 1120 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1587 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 58 CCACAGGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1214 CCCGGG C^CCGGG
XmaI 1214 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 1504 CGCG CG^CG
1506 CGCG
AcyI 461 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
AvaI 93 CTCGGG C^[CT]CG[GA]G
1214 CCCGGG
BsaHI 461 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
BsgI 232 CTGCAC GTGCAG(16/14)
663 GTGCAG
BsiEI 880 CGGCCG CG[GA][CT]^CG
2099 CGGCCG
BsmBI 310 GAGACG CGTCTC(1/5)
459 GAGACG
BsmI 164 GCATTC GAATGC(1/-1)
275 GCATTC
BsoBI 93 CTCGGG C^[CT]CG[GA]G
1214 CCCGGG
BsrFI 434 ACCGGT [GA]^CCGG[CT]
2222 ACCGGC
BstUI 1504 CGCG CG^CG
1506 CGCG
Cfr10I 434 ACCGGT [GA]^CCGG[CT]
2222 ACCGGC
DraIII 195 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1926 CACCCTGTG
EagI 880 CGGCCG C^GGCCG
2099 CGGCCG
EclXI 880 CGGCCG C^GGCCG
2099 CGGCCG
Eco52I 880 CGGCCG C^GGCCG
2099 CGGCCG
Hin1I 461 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
MvnI 1504 CGCG CG^CG
1506 CGCG
NcoI 305 CCATGG C^CATGG
1721 CCATGG
NspI 1553 ACATGC [GA]CATG^[CT]
1736 GCATGT
PleI 622 GACTC GAGTC(4/5)
800 GAGTC
SapI 909 GAAGAGC GCTCTTC(1/4)
1623 GCTCTTC
SmlI 208 CTTGAG C^T[CT][GA]AG
444 CTCAAG
TaqI 290 TCGA T^CGA
1610 TCGA
TthHB8I 290 TCGA T^CGA
1610 TCGA
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PpuMI PstI
RsaI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I TspRI