Restriction map for
pFN21AE9525
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 800 CGCG CG^CG
AflIII 617 ACGTGT A^C[GA][CT]GT
AvaI 640 CCCGGG C^[CT]CG[GA]G
AvaII 844 GGACC G^G[AT]CC
BamHI 269 GGATCC G^GATCC
BfaI 298 CTAG C^TAG
BsgI 646 GTGCAG GTGCAG(16/14)
BsiEI 287 CGGTCG CG[GA][CT]^CG
BsmBI 396 CGTCTC CGTCTC(1/5)
BsmFI 843 GGGAC GGGAC(10/14)
BsoBI 640 CCCGGG C^[CT]CG[GA]G
BsrBI 740 GAGCGG CCGCTC(-3/-3)
BstAPI 862 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 672 GGTCACC G^GT[ACGT]ACC
BstPI 672 GGTCACC G^GT[ACGT]ACC
BstUI 800 CGCG CG^CG
BstXI 339 CCAGGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 588 CCACGG C^C[GA][CT]GG
EcoNI 443 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 672 GGTCACC G^GT[ACGT]ACC
HaeII 1045 AGCGCC [GA]GCGC^[CT]
HincII 1021 GTTGAC GT[CT]^[GA]AC
HindII 1021 GTTGAC GT[CT]^[GA]AC
MaeI 298 CTAG C^TAG
MaeII 617 ACGT A^CGT
MslI 804 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 800 CGCG CG^CG
NaeI 918 GCCGGC GCC^GGC
NgoMIV 918 GCCGGC G^CCGGC
NspI 862 GCATGC [GA]CATG^[CT]
PflMI 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 763 GAGTC GAGTC(4/5)
PpuMI 843 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 338 ACCAGGT A^CC[AT]GGT
SmaI 640 CCCGGG CCC^GGG
SphI 862 GCATGC GCATG^C
TfiI 769 GAATC G^A[AT]TC
Tsp509I 130 AATT ^AATT
Van91I 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 734 CCATCCGAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 640 CCCGGG C^CCGGG
XmaI 640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 168 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
576 CAGGTACTG
BbsI 597 GAAGAC GAAGAC(2/6)
202 GTCTTC
BcnI 640 CCCGG CC^[GC]GG
641 CCGGG
BglI 496 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCCTGCCGGC
BpmI 1041 CTCCAG CTGGAG(16/14)
382 CTGGAG
BpuAI 597 GAAGAC GAAGAC(2/6)
202 GTCTTC
BspMI 676 ACCTGC ACCTGC(4/8)
575 GCAGGT
BsrDI 516 CATTGC GCAATG(2/0)
479 GCAATG
BsrFI 918 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
BstBI 118 TTCGAA TT^CGAA
986 TTCGAA
BstYI 269 GGATCC [GA]^GATC[CT]
530 GGATCT
Cfr10I 918 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
DraII 111 AGGCCCC [GA]G^G[ACGT]CC[CT]
843 GGGACCC
EcoO109I 111 AGGCCCC [GA]G^G[ACGT]CC[CT]
843 GGGACCC
FauI 258 CCCGC CCCGC(4/6)
555 CCCGC
HgaI 798 GACGC GACGC(5/10)
833 GCGTC
HinfI 763 GAGTC G^A[ACGT]TC
769 GAATC
MflI 269 GGATCC [GA]^GATC[CT]
530 GGATCT
MspA1I 259 CCGCTG C[AC]G^C[GT]G
624 CAGCGG
NciI 640 CCCGG CC^[GC]GG
641 CCGGG
NspV 118 TTCGAA TT^CGAA
986 TTCGAA
SfuI 118 TTCGAA TT^CGAA
986 TTCGAA
TaqI 119 TCGA T^CGA
987 TCGA
TthHB8I 119 TCGA T^CGA
987 TCGA
XhoII 269 GGATCC [GA]^GATC[CT]
530 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BbvI BsaI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI