##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        Bsp1286I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccI           74  GTAGAC               GT^[AC][GT]AC
AfaI           81  GTAC                 GT^AC
AluI           48  AGCT                 AG^CT
AvaI           50  CTCGGG               C^[CT]CG[GA]G
AvaII         196  GGACC                G^G[AT]CC
BsaI           56  GAGACC               GGTCTC(1/5)
BsaJI          39  CCTGGG               C^C[ACGT][ACGT]GG
BsmFI         195  GGGAC                GGGAC(10/14)
BsoBI          50  CTCGGG               C^[CT]CG[GA]G
Bsp1407I       80  TGTACA               T^GTACA
BsrGI          80  TGTACA               T^GTACA
BssSI         119  CACGAG               CACGAG(-5/-1)
Cfr13I        196  GGACC                G^G[ACGT]CC
DraII         195  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      195  GGGACCC              [GA]G^G[ACGT]CC[CT]
HaeIII         23  GGCC                 GG^CC
HphI           99  GGTGA                GGTGA(8/7)
MboII         102  GAAGA                GAAGA(8/7)
MwoI            7  GCGACTACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI         195  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI          88  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI           81  GTAC                 GT^AC
Sau96I        196  GGACC                G^G[ACGT]CC
SexAI          59  ACCAGGT              A^CC[AT]GGT
SfcI           11  CTACAG               C^T[GA][CT]AG
SspBI          80  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BpmI          126  CTCCAG               CTGGAG(16/14)
               69  CTGGAG              
BsmAI          56  GAGAC                GTCTC(1/5)
               86  GAGAC               
BsrI           68  ACTGG                ACTGG(1/-1)
               91  ACTGG               
MnlI          125  CCTC                 CCTC(7/6)
              165  GAGG                
TspRI          33  CACTG                CA[GC]TG[ACGT][ACGT]^
               90  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BssKI       BstNI       Cac8I       EcoRII      MvaI        
NlaIV       ScrFI