Restriction map for
pFN21AE5777
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII BamHI BbrPI BciVI
BclI BfrI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FbaI FseI FspI HgaI HindIII
KpnI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI ScaI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1251 GACGTC GACGT^C
AhdI 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 135 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1084 CCTAGG C^CTAGG
BbeI 1076 GGCGCC GGCGC^C
BbvCI 1293 CCTCAGC CCTCAGC(-5/-2)
BglII 287 AGATCT A^GATCT
BlnI 1084 CCTAGG C^CTAGG
BlpI 353 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 353 GCTTAGC GC^T[ACGT]AGC
BsgI 1153 GTGCAG GTGCAG(16/14)
BsrDI 1113 CATTGC GCAATG(2/0)
Bst1107I 1882 GTATAC GTA^TAC
BstZ17I 1882 GTATAC GTA^TAC
CelII 353 GCTTAGC GC^T[ACGT]AGC
Eam1105I 1822 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 400 ATGCAT ATGCA^T
HpaI 793 GTTAAC GTT^AAC
KasI 1076 GGCGCC G^GCGCC
MfeI 896 CAATTG C^AATTG
MspA1I 1273 CAGCTG C[AC]G^C[GT]G
MunI 896 CAATTG C^AATTG
NarI 1076 GGCGCC GG^CGCC
NdeI 1888 CATATG CA^TATG
NsiI 400 ATGCAT ATGCA^T
NspI 14 GCATGC [GA]CATG^[CT]
PstI 881 CTGCAG CTGCA^G
PvuII 1273 CAGCTG CAG^CTG
SalI 1819 GTCGAC G^TCGAC
SexAI 593 ACCTGGT A^CC[AT]GGT
SfoI 1076 GGCGCC GGC^GCC
SmlI 1509 CTTGAG C^T[CT][GA]AG
SphI 14 GCATGC GCATG^C
StuI 584 AGGCCT AGG^CCT
XcmI 1533 CCATGGCCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 135 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 60 CGCG CG^CG
481 CGCG
AcyI 1076 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
AfaI 516 GTAC GT^AC
1667 GTAC
AlwI 33 GATCC GGATC(4/5)
555 GGATC
AspI 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACTACGTC
BanI 238 GGTGCC G^G[CT][GA]CC
1076 GGCGCC
BanII 1512 GAGCTC G[GA]GC[CT]^C
1690 GAGCTC
BbsI 625 GAAGAC GAAGAC(2/6)
1177 GAAGAC
BcgI 1623 CGACTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1448 GCAACCGTGTCG
BfaI 722 CTAG C^TAG
1085 CTAG
BpmI 1030 CTGGAG CTGGAG(16/14)
1337 CTGGAG
BpuAI 625 GAAGAC GAAGAC(2/6)
1177 GAAGAC
BsaHI 1076 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
BseRI 108 CTCCTC GAGGAG(10/8)
676 GAGGAG
BssSI 273 CACGAG CACGAG(-5/-1)
997 CTCGTG
BstUI 60 CGCG CG^CG
481 CGCG
BstXI 1464 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1527 CCATGGCCATGG
BstYI 287 AGATCT [GA]^GATC[CT]
555 GGATCT
EcoRV 852 GATATC GAT^ATC
985 GATATC
FauI 1118 CCCGC CCCGC(4/6)
945 GCGGG
HaeII 1076 GGCGCC [GA]GCGC^[CT]
1523 AGCGCC
Hin1I 1076 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
HincII 793 GTTAAC GT[CT]^[GA]AC
1819 GTCGAC
HindII 793 GTTAAC GT[CT]^[GA]AC
1819 GTCGAC
MaeI 722 CTAG C^TAG
1085 CTAG
MflI 287 AGATCT [GA]^GATC[CT]
555 GGATCT
MvnI 60 CGCG CG^CG
481 CGCG
PflFI 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACTACGTC
PflMI 1131 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1750 CCAAGCCCTGG
RsaI 516 GTAC GT^AC
1667 GTAC
SacI 1512 GAGCTC GAGCT^C
1690 GAGCTC
SfiI 1269 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1323 GGCCCCCCTGGCC
Tth111I 1180 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1804 GACTACGTC
Van91I 1131 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1750 CCAAGCCCTGG
XhoII 287 AGATCT [GA]^GATC[CT]
555 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AluI AlwNI
ApoI AspHI AvaII BalI BbvI
BcnI BglI BmyI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I