Restriction map for
pFN21AE4136
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AclI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BfaI
BglI BglII BlnI BsaAI BsaI
BsiWI BsmBI BspHI BssHII Bst1107I
BstBI BstZ17I Bsu36I CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 997 GTCTAC GT^[AC][GT]AC
AccII 1315 CGCG CG^CG
AccIII 1566 TCCGGA T^CCGGA
AfaI 930 GTAC GT^AC
AflII 750 CTTAAG C^TTAAG
AflIII 932 ACATGT A^C[GA][CT]GT
AgeI 427 ACCGGT A^CCGGT
AhdI 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1382 GTGCAC G^TGCAC
ApaLI 1382 GTGCAC G^TGCAC
AspEI 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 661 GGATCC G^GATCC
BanI 1281 GGTGCC G^G[CT][GA]CC
BbsI 589 GAAGAC GAAGAC(2/6)
BfrI 750 CTTAAG C^TTAAG
BpuAI 589 GAAGAC GAAGAC(2/6)
BsaBI 844 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1566 TCCGGA T^CCGGA
BsiEI 129 CGACCG CG[GA][CT]^CG
BsmFI 1105 GTCCC GGGAC(10/14)
BsmI 11 GCATTC GAATGC(1/-1)
Bsp1407I 929 TGTACA T^GTACA
BspDI 150 ATCGAT AT^CGAT
BspEI 1566 TCCGGA T^CCGGA
BspLU11I 932 ACATGT A^CATGT
BsrBI 1547 GAGCGG CCGCTC(-3/-3)
BsrDI 1583 CATTGC GCAATG(2/0)
BsrFI 427 ACCGGT [GA]^CCGG[CT]
BsrGI 929 TGTACA T^GTACA
BssSI 211 CTCGTG CACGAG(-5/-1)
BstEII 1552 GGTAACC G^GT[ACGT]ACC
BstPI 1552 GGTAACC G^GT[ACGT]ACC
BstUI 1315 CGCG CG^CG
BstXI 1127 CCACTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 427 ACCGGT [GA]^CCGG[CT]
ClaI 150 ATCGAT AT^CGAT
DraI 957 TTTAAA TTT^AAA
DsaI 543 CCGTGG C^C[GA][CT]GG
Eam1105I 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1552 GGTAACC G^GT[ACGT]ACC
EcoRI 235 GAATTC G^AATTC
HaeII 488 GGCGCT [GA]GCGC^[CT]
HphI 363 GGTGA GGTGA(8/7)
MaeII 458 ACGT A^CGT
MamI 844 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1566 TCCGGA T^CCGGA
MspA1I 1502 CAGCTG C[AC]G^C[GT]G
MvnI 1315 CGCG CG^CG
NspI 932 ACATGT [GA]CATG^[CT]
PinAI 427 ACCGGT A^CCGGT
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1502 CAGCTG CAG^CTG
RsaI 930 GTAC GT^AC
SacI 251 GAGCTC GAGCT^C
SspBI 929 TGTACA T^GTACA
Tsp45I 896 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 548 GGCGTC G[GA]^CG[CT]C
1621 GGCGTC
AspHI 251 GAGCTC G[AT]GC[AT]^C
1382 GTGCAC
BalI 215 TGGCCA TGG^CCA
645 TGGCCA
BclI 819 TGATCA T^GATCA
1215 TGATCA
BlpI 580 GCTGAGC GC^T[ACGT]AGC
1309 GCTAAGC
BpmI 172 CTGGAG CTGGAG(16/14)
1030 CTGGAG
Bpu1102I 580 GCTGAGC GC^T[ACGT]AGC
1309 GCTAAGC
BsaHI 548 GGCGTC G[GA]^CG[CT]C
1621 GGCGTC
BsaWI 427 ACCGGT [AT]^CCGG[AT]
1566 TCCGGA
BsiHKAI 251 GAGCTC G[AT]GC[AT]^C
1382 GTGCAC
BsmAI 193 GAGAC GTCTC(1/5)
680 GTCTC
BspMI 411 GCAGGT ACCTGC(4/8)
1302 GCAGGT
BstAPI 609 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
789 GCAGCGAGTGC
CelII 580 GCTGAGC GC^T[ACGT]AGC
1309 GCTAAGC
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
1046 AGGCCCT
EcoNI 348 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1272 CCTCTTCCAGG
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
1046 AGGCCCT
EcoT14I 278 CCTTGG C^C[AT][AT]GG
1139 CCTTGG
FbaI 819 TGATCA T^GATCA
1215 TGATCA
Hin1I 548 GGCGTC G[GA]^CG[CT]C
1621 GGCGTC
MaeIII 896 GTGAC ^GT[ACGT]AC
1553 GTAAC
MfeI 641 CAATTG C^AATTG
1082 CAATTG
MluNI 215 TGGCCA TGG^CCA
645 TGGCCA
MscI 215 TGGCCA TGG^CCA
645 TGGCCA
MseI 751 TTAA T^TAA
958 TTAA
MslI 1389 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1531 CATGACGATG
MunI 641 CAATTG C^AATTG
1082 CAATTG
StyI 278 CCTTGG C^C[AT][AT]GG
1139 CCTTGG
TaqI 151 TCGA T^CGA
552 TCGA
TfiI 50 GAATC G^A[AT]TC
907 GATTC
Tru9I 751 TTAA T^TAA
958 TTAA
TthHB8I 151 TCGA T^CGA
552 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI TseI
Tsp509I TspRI XhoII XmaCI XmaI