Restriction map for
pFN21AE5252
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MluI MluNI MnlI MroI MscI
MspA1I MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 313 GTAC GT^AC
AlwI 142 GATCC GGATC(4/5)
BbvI 376 GCTGC GCAGC(8/12)
BlpI 361 GCTAAGC GC^T[ACGT]AGC
BpmI 356 CTGGAG CTGGAG(16/14)
Bpu1102I 361 GCTAAGC GC^T[ACGT]AGC
BsaJI 112 CCATGG C^C[ACGT][ACGT]GG
BspHI 440 TCATGA T^CATGA
BspMI 285 GCAGGT ACCTGC(4/8)
BsrI 45 CCAGT ACTGG(1/-1)
BstF5I 42 CATCC GGATG(2/0)
BstYI 141 AGATCC [GA]^GATC[CT]
Cac8I 420 GCATGC GC[ACGT]^[ACGT]GC
CelII 361 GCTAAGC GC^T[ACGT]AGC
Cfr13I 226 GGGCC G^G[ACGT]CC
DpnII 142 GATC ^GATC
DraI 507 TTTAAA TTT^AAA
DraII 225 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 112 CCATGG C^C[GA][CT]GG
EcoO109I 225 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 112 CCATGG C^C[AT][AT]GG
Fnu4HI 376 GCTGC GC^[ACGT]GC
FokI 42 CATCC GGATG(9/13)
HaeIII 227 GGCC GG^CC
HincII 472 GTCAAC GT[CT]^[GA]AC
HindII 472 GTCAAC GT[CT]^[GA]AC
HindIII 364 AAGCTT A^AGCTT
ItaI 376 GCTGC GC^[ACGT]GC
MboI 142 GATC ^GATC
MfeI 82 CAATTG C^AATTG
MflI 141 AGATCC [GA]^GATC[CT]
MslI 441 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 82 CAATTG C^AATTG
NcoI 112 CCATGG C^CATGG
NdeI 526 CATATG CA^TATG
NdeII 142 GATC ^GATC
NlaIV 298 GGAGCC GG[ACGT]^[ACGT]CC
NspI 420 GCATGC [GA]CATG^[CT]
PleI 437 GAGTC GAGTC(4/5)
RcaI 440 TCATGA T^CATGA
RsaI 313 GTAC GT^AC
Sau3AI 142 GATC ^GATC
Sau96I 226 GGGCC G^G[ACGT]CC
SphI 420 GCATGC GCATG^C
StyI 112 CCATGG C^C[AT][AT]GG
SwaI 506 ATTTAAAT ATTT^AAAT
TfiI 78 GAATC G^A[AT]TC
TseI 376 GCTGC G^C[AT]GC
TspRI 318 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 141 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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AcsI 10 AAATTT [GA]^AATT[CT]
307 AAATTT
AluI 360 AGCT AG^CT
365 AGCT
ApoI 10 AAATTT [GA]^AATT[CT]
307 AAATTT
BbsI 457 GAAGAC GAAGAC(2/6)
531 GAAGAC
BpuAI 457 GAAGAC GAAGAC(2/6)
531 GAAGAC
HinfI 78 GAATC G^A[ACGT]TC
437 GAGTC
MwoI 276 GCCACACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
397 GCTATCATGGC
PstI 377 CTGCAG CTGCA^G
452 CTGCAG
SfaNI 161 GATGC GCATC(5/9)
41 GCATC
SfcI 377 CTGCAG C^T[GA][CT]AG
452 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BssKI BstNI DdeI EcoRII MboII
MseI MvaI NlaIII ScrFI Tru9I
Tsp509I