Restriction map for
pFN21AE2309
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AviII
AvrII BciVI BfrI BlnI BsaBI
BsiWI BsmBI Bsp1407I BspDI BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
DraI DraIII Eco47III EcoNI EcoRI
EcoRV FseI FspI HindIII MamI
MluI NdeI NheI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 488 GGTACC G^GTACC
AccI 3142 GTCGAC GT^[AC][GT]AC
AccIII 3636 TCCGGA T^CCGGA
AflIII 2336 ACATGT A^C[GA][CT]GT
AhdI 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 488 GGTACC G^GTACC
AspEI 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1031 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 440 TGATCA T^GATCA
BglII 2928 AGATCT A^GATCT
BsaWI 3636 TCCGGA [AT]^CCGG[AT]
BseAI 3636 TCCGGA T^CCGGA
BsmI 1996 GAATGC GAATGC(1/-1)
BspEI 3636 TCCGGA T^CCGGA
BspHI 2634 TCATGA T^CATGA
BspLU11I 2336 ACATGT A^CATGT
BssHII 629 GCGCGC G^CGCGC
BstAPI 3580 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 87 GGTGACC G^GT[ACGT]ACC
BstPI 87 GGTGACC G^GT[ACGT]ACC
CpoI 906 CGGTCCG CG^G[AT]CCG
Eam1105I 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
EcoT22I 958 ATGCAT ATGCA^T
FbaI 440 TGATCA T^GATCA
HpaI 1053 GTTAAC GTT^AAC
KpnI 488 GGTACC GGTAC^C
MfeI 1473 CAATTG C^AATTG
MroI 3636 TCCGGA T^CCGGA
MunI 1473 CAATTG C^AATTG
NotI 391 GCGGCCGC GC^GGCCGC
NsiI 958 ATGCAT ATGCA^T
RcaI 2634 TCATGA T^CATGA
RsrII 906 CGGTCCG CG^G[AT]CCG
SalI 3142 GTCGAC G^TCGAC
SmaI 3429 CCCGGG CCC^GGG
XbaI 1636 TCTAGA T^CTAGA
XmaCI 3429 CCCGGG C^CCGGG
XmaI 3429 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AlwNI 832 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3452 CAGCTGCTG
AocI 1146 CCTCAGG CC^T[ACGT]AGG
2386 CCTCAGG
AspI 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2758 GACTACGTC
BamHI 1582 GGATCC G^GATCC
1795 GGATCC
BbeI 403 GGCGCC GGCGC^C
1042 GGCGCC
BbrPI 2948 CACGTG CAC^GTG
3712 CACGTG
BbvCI 3344 CCTCAGC CCTCAGC(-5/-2)
3387 CCTCAGC
Bsu36I 1146 CCTCAGG CC^T[ACGT]AGG
2386 CCTCAGG
EagI 392 CGGCCG C^GGCCG
650 CGGCCG
EclXI 392 CGGCCG C^GGCCG
650 CGGCCG
Eco52I 392 CGGCCG C^GGCCG
650 CGGCCG
Eco81I 1146 CCTCAGG CC^T[ACGT]AGG
2386 CCTCAGG
HgaI 310 GACGC GACGC(5/10)
3248 GACGC
KasI 403 GGCGCC G^GCGCC
1042 GGCGCC
KspI 371 CCGCGG CCGC^GG
430 CCGCGG
NaeI 648 GCCGGC GCC^GGC
1284 GCCGGC
NarI 403 GGCGCC GG^CGCC
1042 GGCGCC
NcoI 1737 CCATGG C^CATGG
3083 CCATGG
NgoMIV 648 GCCGGC G^CCGGC
1284 GCCGGC
NruI 21 TCGCGA TCG^CGA
530 TCGCGA
PflFI 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2758 GACTACGTC
PflMI 1912 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2405 CCAAAAGTTGG
PmaCI 2948 CACGTG CAC^GTG
3712 CACGTG
PmlI 2948 CACGTG CAC^GTG
3712 CACGTG
SacII 371 CCGCGG CCGC^GG
430 CCGCGG
SapI 3232 GAAGAGC GCTCTTC(1/4)
2396 GCTCTTC
SfoI 403 GGCGCC GGC^GCC
1042 GGCGCC
Tth111I 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2758 GACTACGTC
Van91I 1912 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2405 CCAAAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII