Restriction map for
pFN21AE3187
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiEI BsmBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PstI PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 252 GGTACC G^GTACC
AcyI 417 GGCGTC G[GA]^CG[CT]C
ApaI 265 GGGCCC GGGCC^C
Asp718I 252 GGTACC G^GTACC
AspHI 713 GAGCTC G[AT]GC[AT]^C
BbrPI 1138 CACGTG CAC^GTG
BbsI 1237 GAAGAC GAAGAC(2/6)
BciVI 1024 GTATCC GTATCC(6/5)
BlpI 192 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC
BpuAI 1237 GAAGAC GAAGAC(2/6)
BsaAI 1138 CACGTG [CT]AC^GT[GA]
BsaHI 417 GGCGTC G[GA]^CG[CT]C
BsiHKAI 713 GAGCTC G[AT]GC[AT]^C
BsiWI 178 CGTACG C^GTACG
BsmI 705 GAATGC GAATGC(1/-1)
BspHI 506 TCATGA T^CATGA
BspLU11I 904 ACATGT A^CATGT
BsrDI 455 GCAATG GCAATG(2/0)
BstXI 53 CCAGATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 392 AGATCC [GA]^GATC[CT]
CelII 192 GCTGAGC GC^T[ACGT]AGC
CfoI 274 GCGC GCG^C
EaeI 213 CGGCCA [CT]^GGCC[GA]
EarI 1060 GAAGAG CTCTTC(1/4)
HaeII 273 GGCGCT [GA]GCGC^[CT]
HgaI 418 GCGTC GACGC(5/10)
HhaI 274 GCGC GCG^C
Hin1I 417 GGCGTC G[GA]^CG[CT]C
HinP1I 274 GCGC G^CGC
HindIII 968 AAGCTT A^AGCTT
KpnI 252 GGTACC GGTAC^C
Ksp632I 1060 GAAGAG CTCTTC(1/4)
KspI 33 CCGCGG CCGC^GG
MflI 392 AGATCC [GA]^GATC[CT]
NcoI 916 CCATGG C^CATGG
NspI 904 ACATGT [GA]CATG^[CT]
PflMI 314 CCACGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1138 CACGTG CAC^GTG
PmlI 1138 CACGTG CAC^GTG
PshAI 567 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1165 CAGCTG CAG^CTG
RcaI 506 TCATGA T^CATGA
SacI 713 GAGCTC GAGCT^C
SacII 33 CCGCGG CCGC^GG
SfcI 1162 CTACAG C^T[GA][CT]AG
TfiI 609 GAATC G^A[AT]TC
Van91I 314 CCACGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 392 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 490 GTAGAC GT^[AC][GT]AC
1160 GTCTAC
AlwI 393 GATCC GGATC(4/5)
1000 GGATC
BanII 265 GGGCCC G[GA]GC[CT]^C
713 GAGCTC
BglI 32 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
258 GCCCGACGGGC
BsaI 1078 GAGACC GGTCTC(1/5)
24 GGTCTC
BseRI 1050 CTCCTC GAGGAG(10/8)
199 GAGGAG
BsmFI 448 GGGAC GGGAC(10/14)
1018 GTCCC
DdeI 85 CTGAG C^T[ACGT]AG
193 CTGAG
DraII 245 GGGTCCT [GA]G^G[ACGT]CC[CT]
448 GGGACCC
EcoO109I 245 GGGTCCT [GA]G^G[ACGT]CC[CT]
448 GGGACCC
EcoT14I 601 CCTTGG C^C[AT][AT]GG
916 CCATGG
HphI 606 GGTGA GGTGA(8/7)
477 TCACC
PleI 220 GACTC GAGTC(4/5)
87 GAGTC
PpuMI 245 GGGTCCT [GA]G^G[AT]CC[CT]
448 GGGACCC
StyI 601 CCTTGG C^C[AT][AT]GG
916 CCATGG
TaqI 693 TCGA T^CGA
1269 TCGA
Tsp45I 565 GTGAC ^GT[GC]AC
956 GTGAC
TthHB8I 693 TCGA T^CGA
1269 TCGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI AvaI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI Tru9I TseI Tsp509I
TspRI XmaCI XmaI