Restriction map for
pFN21AE5542
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MseI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1028 GGTACC G^GTACC
AflIII 1022 ACATGT A^C[GA][CT]GT
AlwI 817 GGATC GGATC(4/5)
Asp700I 33 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1028 GGTACC G^GTACC
AvaI 1058 CTCGGG C^[CT]CG[GA]G
AvaII 678 GGACC G^G[AT]CC
BanI 1028 GGTACC G^G[CT][GA]CC
BcgI 427 CGAAGAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 705 CCGGG CC^[GC]GG
BglI 731 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 823 GAGACC GGTCTC(1/5)
BsaJI 1057 CCTCGG C^C[ACGT][ACGT]GG
BsaWI 126 TCCGGT [AT]^CCGG[AT]
BsgI 663 GTGCAG GTGCAG(16/14)
BsiEI 738 CGGCCG CG[GA][CT]^CG
BsmFI 115 GTCCC GGGAC(10/14)
BsoBI 1058 CTCGGG C^[CT]CG[GA]G
BspLU11I 1022 ACATGT A^CATGT
BsrDI 980 CATTGC GCAATG(2/0)
BstYI 817 GGATCT [GA]^GATC[CT]
CfoI 268 GCGC GCG^C
EaeI 738 CGGCCG [CT]^GGCC[GA]
EagI 738 CGGCCG C^GGCCG
EarI 634 GAAGAG CTCTTC(1/4)
EclXI 738 CGGCCG C^GGCCG
Eco52I 738 CGGCCG C^GGCCG
EcoNI 1057 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 90 GAATTC G^AATTC
HhaI 268 GCGC GCG^C
HinP1I 268 GCGC G^CGC
HincII 766 GTTGAC GT[CT]^[GA]AC
HindII 766 GTTGAC GT[CT]^[GA]AC
KpnI 1028 GGTACC GGTAC^C
Ksp632I 634 GAAGAG CTCTTC(1/4)
MaeII 962 ACGT A^CGT
MfeI 105 CAATTG C^AATTG
MflI 817 GGATCT [GA]^GATC[CT]
MslI 1011 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MunI 105 CAATTG C^AATTG
NciI 705 CCGGG CC^[GC]GG
PvuII 517 CAGCTG CAG^CTG
SapI 634 GAAGAGC GCTCTTC(1/4)
ScaI 875 AGTACT AGT^ACT
SfaNI 312 GATGC GCATC(5/9)
SphI 157 GCATGC GCATG^C
XcmI 578 CCAAAAATAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 817 GGATCT [GA]^GATC[CT]
XmnI 33 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BfaI 179 CTAG C^TAG
646 CTAG
BpmI 367 CTCCAG CTGGAG(16/14)
589 CTGGAG
BspMI 920 ACCTGC ACCTGC(4/8)
1045 ACCTGC
BstAPI 846 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
863 GCAACTTGTGC
BstXI 516 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1010 CCATCCTGGTGG
HapII 127 CCGG C^CGG
705 CCGG
HpaII 127 CCGG C^CGG
705 CCGG
HphI 615 GGTGA GGTGA(8/7)
1004 TCACC
MaeI 179 CTAG C^TAG
646 CTAG
MspI 127 CCGG C^CGG
705 CCGG
NspI 157 GCATGC [GA]CATG^[CT]
1022 ACATGT
PleI 967 GACTC GAGTC(4/5)
592 GAGTC
PstI 853 CTGCAG CTGCA^G
922 CTGCAG
SfcI 853 CTGCAG C^T[GA][CT]AG
922 CTGCAG
TaqI 426 TCGA T^CGA
632 TCGA
Tsp45I 46 GTCAC ^GT[GC]AC
404 GTGAC
TthHB8I 426 TCGA T^CGA
632 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I TspRI