Restriction map for
pFN21AE4004
##################################
# NO CUT ENZYMES ( 195 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspI MunI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 24 CGCG CG^CG
AluI 101 AGCT AG^CT
Alw44I 106 GTGCAC G^TGCAC
AlwNI 97 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 106 GTGCAC G^TGCAC
AspHI 106 GTGCAC G[AT]GC[AT]^C
AvaII 45 GGACC G^G[AT]CC
BbvCI 175 GCTGAGG CCTCAGC(-5/-2)
BmyI 106 GTGCAC G[AGT]GC[ACT]^C
BsgI 38 GTGCAG GTGCAG(16/14)
BsiHKAI 106 GTGCAC G[AT]GC[AT]^C
BsiYI 96 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 96 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 76 GAGAC GTCTC(1/5)
Bsp1286I 106 GTGCAC G[AGT]GC[ACT]^C
BssKI 151 CCTGG ^CC[ACGT]GG
BstAPI 99 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 151 CCTGG CC^[AT]GG
BstUI 24 CGCG CG^CG
DsaI 23 CCGCGG C^C[GA][CT]GG
EarI 15 CTCTTC CTCTTC(1/4)
EcoRII 151 CCTGG ^CC[AT]GG
Ksp632I 15 CTCTTC CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MboII 16 TCTTC GAAGA(8/7)
MvaI 151 CCTGG CC^[AT]GG
MvnI 24 CGCG CG^CG
NlaIII 141 CATG CATG^
NlaIV 45 GGACCC GG[ACGT]^[ACGT]CC
PflMI 96 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 78 GACTC GAGTC(4/5)
PvuII 100 CAGCTG CAG^CTG
SacII 23 CCGCGG CCGC^GG
ScrFI 151 CCTGG CC^[ACGT]GG
StuI 179 AGGCCT AGG^CCT
TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 96 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Aor51HI 89 AGCGCT AGC^GCT
203 AGCGCT
BsaJI 23 CCGCGG C^C[ACGT][ACGT]GG
151 CCTGGG
BseRI 9 CTCCTC GAGGAG(10/8)
12 CTCCTC
CfoI 90 GCGC GCG^C
204 GCGC
Cfr13I 45 GGACC G^G[ACGT]CC
154 GGGCC
DdeI 176 CTGAG C^T[ACGT]AG
273 CTCAG
Eco47III 89 AGCGCT AGC^GCT
203 AGCGCT
FauI 48 CCCGC CCCGC(4/6)
28 GCGGG
HaeII 89 AGCGCT [GA]GCGC^[CT]
203 AGCGCT
HaeIII 155 GGCC GG^CC
180 GGCC
HhaI 90 GCGC GCG^C
204 GCGC
HinP1I 90 GCGC G^CGC
204 GCGC
MspA1I 23 CCGCGG C[AC]G^C[GT]G
100 CAGCTG
Sau96I 45 GGACC G^G[ACGT]CC
154 GGGCC
TaqI 68 TCGA T^CGA
237 TCGA
TfiI 4 GATTC G^A[AT]TC
234 GAATC
TthHB8I 68 TCGA T^CGA
237 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BbvI BsrI Fnu4HI HinfI
ItaI MnlI MwoI TseI