Restriction map for
pFN21AE6586
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BanI
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsgI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MluI MroI MseI
MslI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 21 CGCG CG^CG
AcyI 260 GACGCC G[GA]^CG[CT]C
AluI 69 AGCT AG^CT
AvaI 47 CTCGGG C^[CT]CG[GA]G
BamHI 243 GGATCC G^GATCC
BanII 44 GGGCTC G[GA]GC[CT]^C
BbrPI 412 CACGTG CAC^GTG
BpmI 71 CTGGAG CTGGAG(16/14)
BsaAI 412 CACGTG [CT]AC^GT[GA]
BsaHI 260 GACGCC G[GA]^CG[CT]C
BsaWI 157 TCCGGT [AT]^CCGG[AT]
BsiEI 159 CGGTCG CG[GA][CT]^CG
BsiYI 58 CCTAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 58 CCTAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 258 GAGAC GTCTC(1/5)
BsmBI 258 GAGACG CGTCTC(1/5)
BsmFI 492 GTCCC GGGAC(10/14)
BsoBI 47 CTCGGG C^[CT]CG[GA]G
BspMI 277 ACCTGC ACCTGC(4/8)
BssSI 302 CTCGTG CACGAG(-5/-1)
BstF5I 52 GGATG GGATG(2/0)
BstUI 21 CGCG CG^CG
BstYI 243 GGATCC [GA]^GATC[CT]
CfoI 374 GCGC GCG^C
DdeI 170 CTAAG C^T[ACGT]AG
DsaI 93 CCATGG C^C[GA][CT]GG
EarI 80 GAAGAG CTCTTC(1/4)
EcoNI 58 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 52 GGATG GGATG(9/13)
HapII 158 CCGG C^CGG
HgaI 260 GACGC GACGC(5/10)
HhaI 374 GCGC GCG^C
Hin1I 260 GACGCC G[GA]^CG[CT]C
HinP1I 374 GCGC G^CGC
HincII 446 GTCAAC GT[CT]^[GA]AC
HindII 446 GTCAAC GT[CT]^[GA]AC
HinfI 154 GAGTC G^A[ACGT]TC
HpaII 158 CCGG C^CGG
HphI 151 GGTGA GGTGA(8/7)
Ksp632I 80 GAAGAG CTCTTC(1/4)
MaeII 413 ACGT A^CGT
MfeI 423 CAATTG C^AATTG
MflI 243 GGATCC [GA]^GATC[CT]
MspA1I 457 CCGCTG C[AC]G^C[GT]G
MspI 158 CCGG C^CGG
MunI 423 CAATTG C^AATTG
MvnI 21 CGCG CG^CG
NcoI 93 CCATGG C^CATGG
NlaIII 94 CATG CATG^
PleI 154 GAGTC GAGTC(4/5)
PmaCI 412 CACGTG CAC^GTG
PmlI 412 CACGTG CAC^GTG
Tsp45I 305 GTGAC ^GT[GC]AC
Tsp509I 424 AATT ^AATT
TspRI 268 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 243 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 244 GATCC GGATC(4/5)
243 GGATC
BbvI 428 GCAGC GCAGC(8/12)
459 GCTGC
BmyI 44 GGGCTC G[AGT]GC[ACT]^C
355 GGGCAC
BseRI 62 GAGGAG GAGGAG(10/8)
65 GAGGAG
Bsp1286I 44 GGGCTC G[AGT]GC[ACT]^C
355 GGGCAC
BssKI 378 CCAGG ^CC[ACGT]GG
442 CCAGG
BstNI 378 CCAGG CC^[AT]GG
442 CCAGG
DpnII 244 GATC ^GATC
313 GATC
EcoRII 378 CCAGG ^CC[AT]GG
442 CCAGG
EcoT14I 93 CCATGG C^C[AT][AT]GG
483 CCAAGG
MboI 244 GATC ^GATC
313 GATC
MboII 80 GAAGA GAAGA(8/7)
17 TCTTC
MvaI 378 CCAGG CC^[AT]GG
442 CCAGG
NdeII 244 GATC ^GATC
313 GATC
NlaIV 115 GGTTCC GG[ACGT]^[ACGT]CC
243 GGATCC
Sau3AI 244 GATC ^GATC
313 GATC
ScrFI 378 CCAGG CC^[ACGT]GG
442 CCAGG
SfaNI 53 GATGC GCATC(5/9)
395 GATGC
StyI 93 CCATGG C^C[AT][AT]GG
483 CCAAGG
TseI 428 GCAGC G^C[AT]GC
459 GCTGC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AciI BalI BfaI BsaJI EaeI
Fnu4HI HaeIII ItaI MaeI MaeIII
MluNI MnlI MscI MwoI