Restriction map for
pFN21AE6693
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 568 GTCTAC GT^[AC][GT]AC
AclI 182 AACGTT AA^CGTT
AcyI 321 GACGCC G[GA]^CG[CT]C
AfaI 364 GTAC GT^AC
AflIII 366 ACGTGT A^C[GA][CT]GT
AlwNI 614 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 818 CCCGAG C^[CT]CG[GA]G
BanII 469 GAGCTC G[GA]GC[CT]^C
BcgI 732 CGATTTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 475 CTAG C^TAG
BglI 878 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 50 AGATCT A^GATCT
BsaAI 365 TACGTG [CT]AC^GT[GA]
BsaHI 321 GACGCC G[GA]^CG[CT]C
BsoBI 818 CCCGAG C^[CT]CG[GA]G
BspDI 730 ATCGAT AT^CGAT
BsrI 514 CCAGT ACTGG(1/-1)
BssSI 653 CTCGTG CACGAG(-5/-1)
BstF5I 306 GGATG GGATG(2/0)
BstYI 50 AGATCT [GA]^GATC[CT]
ClaI 730 ATCGAT AT^CGAT
DdeI 276 CTTAG C^T[ACGT]AG
DpnII 51 GATC ^GATC
DraII 641 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 641 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 668 CCCGC CCCGC(4/6)
FokI 306 GGATG GGATG(9/13)
HgaI 321 GACGC GACGC(5/10)
Hin1I 321 GACGCC G[GA]^CG[CT]C
HincII 295 GTTGAC GT[CT]^[GA]AC
HindII 295 GTTGAC GT[CT]^[GA]AC
HinfI 139 GACTC G^A[ACGT]TC
HphI 44 TCACC GGTGA(8/7)
MaeI 475 CTAG C^TAG
MboI 51 GATC ^GATC
MboII 53 TCTTC GAAGA(8/7)
MflI 50 AGATCT [GA]^GATC[CT]
MseI 727 TTAA T^TAA
MslI 697 CATAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 51 GATC ^GATC
PleI 139 GACTC GAGTC(4/5)
Psp1406I 182 AACGTT AA^CGTT
RsaI 364 GTAC GT^AC
SacI 469 GAGCTC GAGCT^C
Sau3AI 51 GATC ^GATC
SbfI 462 CCTGCAGG CCTGCA^GG
SmlI 255 CTTGAG C^T[CT][GA]AG
Sse8387I 462 CCTGCAGG CCTGCA^GG
TaqI 731 TCGA T^CGA
Tru9I 727 TTAA T^TAA
Tsp45I 834 GTCAC ^GT[GC]AC
TthHB8I 731 TCGA T^CGA
XcmI 899 CCATCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 50 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 427 GTGCTC G[AT]GC[AT]^C
469 GAGCTC
AvaII 153 GGACC G^G[AT]CC
849 GGTCC
BanI 385 GGTGCC G^G[CT][GA]CC
575 GGCACC
BsgI 630 CTGCAC GTGCAG(16/14)
485 GTGCAG
BsiHKAI 427 GTGCTC G[AT]GC[AT]^C
469 GAGCTC
BsiYI 552 CCTCTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
824 CCACTCCACGG
BslI 552 CCTCTATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
824 CCACTCCACGG
BsmFI 220 GGGAC GGGAC(10/14)
658 GTCCC
BsrDI 798 CATTGC GCAATG(2/0)
803 CATTGC
CfoI 165 GCGC GCG^C
231 GCGC
EcoT14I 282 CCATGG C^C[AT][AT]GG
480 CCATGG
HhaI 165 GCGC GCG^C
231 GCGC
HinP1I 165 GCGC G^CGC
231 GCGC
MaeIII 176 GTTAC ^GT[ACGT]AC
834 GTCAC
NcoI 282 CCATGG C^CATGG
480 CCATGG
PstI 64 CTGCAG CTGCA^G
463 CTGCAG
SfaNI 404 GCATC GCATC(5/9)
523 GCATC
StyI 282 CCATGG C^C[AT][AT]GG
480 CCATGG
Tsp509I 17 AATT ^AATT
700 AATT
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BalI BbvI BmyI
BsaJI BseRI Bsp1286I BssKI BstNI
Cac8I Cfr13I DsaI EaeI EcoRII
Fnu4HI HaeIII ItaI MaeII MluNI
MnlI MscI MspA1I MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
SfcI TseI TspRI