Restriction map for
pFN21AE6760
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiWI BsmAI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 801 GTGCAC G^TGCAC
AlwNI 860 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 801 GTGCAC G^TGCAC
Asp700I 1012 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 801 GTGCAC G[AT]GC[AT]^C
BalI 167 TGGCCA TGG^CCA
BpmI 737 CTCCAG CTGGAG(16/14)
BsgI 633 CTGCAC GTGCAG(16/14)
BsiEI 124 CGGCCG CG[GA][CT]^CG
BsiHKAI 801 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1208 TGTACA T^GTACA
BspHI 434 TCATGA T^CATGA
BsrFI 126 GCCGGT [GA]^CCGG[CT]
BsrGI 1208 TGTACA T^GTACA
BstBI 692 TTCGAA TT^CGAA
BstEII 1144 GGTGACC G^GT[ACGT]ACC
BstPI 1144 GGTGACC G^GT[ACGT]ACC
CfoI 46 GCGC GCG^C
Cfr10I 126 GCCGGT [GA]^CCGG[CT]
DraI 903 TTTAAA TTT^AAA
DraII 861 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1149 CCATGG C^C[GA][CT]GG
EagI 124 CGGCCG C^GGCCG
EclXI 124 CGGCCG C^GGCCG
Eco52I 124 CGGCCG C^GGCCG
EcoNI 733 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 861 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1144 GGTGACC G^GT[ACGT]ACC
HaeII 45 GGCGCT [GA]GCGC^[CT]
HapII 127 CCGG C^CGG
HhaI 46 GCGC GCG^C
HinP1I 46 GCGC G^CGC
HpaII 127 CCGG C^CGG
MaeII 239 ACGT A^CGT
MluNI 167 TGGCCA TGG^CCA
MscI 167 TGGCCA TGG^CCA
MspI 127 CCGG C^CGG
NcoI 1149 CCATGG C^CATGG
NdeI 587 CATATG CA^TATG
NspV 692 TTCGAA TT^CGAA
PpuMI 861 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 434 TCATGA T^CATGA
SfuI 692 TTCGAA TT^CGAA
SspBI 1208 TGTACA T^GTACA
TaqI 693 TCGA T^CGA
TthHB8I 693 TCGA T^CGA
XcmI 1500 CCAGGTTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1012 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 916 AAATTT [GA]^AATT[CT]
1126 AAATTT
AflIII 1038 ACATGT A^C[GA][CT]GT
1158 ACATGT
ApoI 916 AAATTT [GA]^AATT[CT]
1126 AAATTT
BanII 669 GGGCTC G[GA]GC[CT]^C
782 GAGCCC
BbsI 829 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BglII 563 AGATCT A^GATCT
972 AGATCT
BpuAI 829 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BspLU11I 1038 ACATGT A^CATGT
1158 ACATGT
BstXI 470 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1223 CCATTGGACTGG
BstYI 563 AGATCT [GA]^GATC[CT]
972 AGATCT
FauI 20 CCCGC CCCGC(4/6)
42 GCGGG
HindIII 316 AAGCTT A^AGCTT
395 AAGCTT
MflI 563 AGATCT [GA]^GATC[CT]
972 AGATCT
MslI 471 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CATTCGAATG
MspA1I 163 CAGCTG C[AC]G^C[GT]G
644 CAGCTG
NspI 1038 ACATGT [GA]CATG^[CT]
1158 ACATGT
PflMI 1500 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1548 CCAGTACTTGG
PvuII 163 CAGCTG CAG^CTG
644 CAGCTG
ScaI 1061 AGTACT AGT^ACT
1550 AGTACT
SfcI 305 CTGTAG C^T[GA][CT]AG
525 CTGTAG
TfiI 230 GAATC G^A[AT]TC
550 GAATC
Van91I 1500 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1548 CCAGTACTTGG
XhoII 563 AGATCT [GA]^GATC[CT]
972 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BclI BfaI BmyI
BsaJI BsiYI BslI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI