Restriction map for
pFN21AE1814
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PleI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 9 GACGTC GACGT^C
AccI 31 GTCTAC GT^[AC][GT]AC
AcsI 103 GAATTC [GA]^AATT[CT]
AcyI 9 GACGTC G[GA]^CG[CT]C
AgeI 364 ACCGGT A^CCGGT
ApoI 103 GAATTC [GA]^AATT[CT]
AvaI 604 CCCGAG C^[CT]CG[GA]G
BbrPI 208 CACGTG CAC^GTG
BlpI 174 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
BsaAI 208 CACGTG [CT]AC^GT[GA]
BsaHI 9 GACGTC G[GA]^CG[CT]C
BsaI 367 GGTCTC GGTCTC(1/5)
BsaWI 364 ACCGGT [AT]^CCGG[AT]
BsmAI 368 GTCTC GTCTC(1/5)
BsoBI 604 CCCGAG C^[CT]CG[GA]G
BspLU11I 432 ACATGT A^CATGT
BsrDI 423 CATTGC GCAATG(2/0)
BsrFI 364 ACCGGT [GA]^CCGG[CT]
CelII 174 GCTGAGC GC^T[ACGT]AGC
CfoI 74 GCGC GCG^C
Cfr10I 364 ACCGGT [GA]^CCGG[CT]
CpoI 737 CGGACCG CG^G[AT]CCG
DraII 716 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 208 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 42 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 716 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 103 GAATTC G^AATTC
HaeII 73 AGCGCC [GA]GCGC^[CT]
HgaI 489 GACGC GACGC(5/10)
HhaI 74 GCGC GCG^C
Hin1I 9 GACGTC G[GA]^CG[CT]C
HinP1I 74 GCGC G^CGC
HincII 136 GTTGAC GT[CT]^[GA]AC
HindII 136 GTTGAC GT[CT]^[GA]AC
HinfI 733 GATTC G^A[ACGT]TC
MseI 314 TTAA T^TAA
MslI 690 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 432 ACATGT [GA]CATG^[CT]
PinAI 364 ACCGGT A^CCGGT
PmaCI 208 CACGTG CAC^GTG
PmlI 208 CACGTG CAC^GTG
PpuMI 716 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 5 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 737 CGGACCG CG^G[AT]CCG
SacI 723 GAGCTC GAGCT^C
SbfI 225 CCTGCAGG CCTGCA^GG
SexAI 246 ACCAGGT A^CC[AT]GGT
Sse8387I 225 CCTGCAGG CCTGCA^GG
TaqI 569 TCGA T^CGA
TfiI 733 GATTC G^A[AT]TC
Tru9I 314 TTAA T^TAA
Tsp509I 104 AATT ^AATT
TthHB8I 569 TCGA T^CGA
XcmI 887 CCATCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 432 ACATGT A^C[GA][CT]GT
902 ACACGT
AlwI 261 GATCC GGATC(4/5)
867 GATCC
AlwNI 169 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
913 CAGCCCCTG
AspHI 65 GTGCTC G[AT]GC[AT]^C
723 GAGCTC
BanII 350 GGGCTC G[GA]GC[CT]^C
723 GAGCTC
BbsI 961 GAAGAC GAAGAC(2/6)
514 GTCTTC
BcnI 480 CCGGG CC^[GC]GG
618 CCCGG
BpmI 522 CTCCAG CTGGAG(16/14)
162 CTGGAG
BpuAI 961 GAAGAC GAAGAC(2/6)
514 GTCTTC
BseRI 403 CTCCTC GAGGAG(10/8)
493 CTCCTC
BsgI 831 CTGCAC GTGCAG(16/14)
146 GTGCAG
BsiHKAI 65 GTGCTC G[AT]GC[AT]^C
723 GAGCTC
BspMI 224 ACCTGC ACCTGC(4/8)
510 GCAGGT
BsrI 93 ACTGG ACTGG(1/-1)
753 ACTGG
BstXI 154 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
669 CCAGCTGGATGG
BstYI 260 AGATCC [GA]^GATC[CT]
866 AGATCC
EarI 112 CTCTTC CTCTTC(1/4)
840 GAAGAG
FauI 686 CCCGC CCCGC(4/6)
24 GCGGG
HphI 275 TCACC GGTGA(8/7)
982 TCACC
Ksp632I 112 CTCTTC CTCTTC(1/4)
840 GAAGAG
MflI 260 AGATCC [GA]^GATC[CT]
866 AGATCC
NciI 480 CCGGG CC^[GC]GG
618 CCCGG
NlaIII 373 CATG CATG^
433 CATG
PflMI 207 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
912 CCAGCCCCTGG
PstI 226 CTGCAG CTGCA^G
508 CTGCAG
SfaNI 566 GCATC GCATC(5/9)
689 GCATC
StuI 572 AGGCCT AGG^CCT
827 AGGCCT
Van91I 207 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
912 CCAGCCCCTGG
XhoII 260 AGATCC [GA]^GATC[CT]
866 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NdeII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I TspRI