Restriction map for
pFN21AB9455
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BlnI
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraII DrdI Eam1105I EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NgoMIV
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 4035 CGCG CG^CG
AflII 1486 CTTAAG C^TTAAG
AflIII 2759 ACATGT A^C[GA][CT]GT
Alw44I 3949 GTGCAC G^TGCAC
AocI 3936 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3497 AGCGCT AGC^GCT
ApaLI 3949 GTGCAC G^TGCAC
AspI 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3325 TGGCCA TGG^CCA
BanI 2815 GGTGCC G^G[CT][GA]CC
BbvCI 3493 CCTCAGC CCTCAGC(-5/-2)
BciVI 2462 GTATCC GTATCC(6/5)
BclI 3716 TGATCA T^GATCA
BfrI 1486 CTTAAG C^TTAAG
BglII 618 AGATCT A^GATCT
BlpI 150 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaI 962 GAGACC GGTCTC(1/5)
BsaWI 3408 TCCGGT [AT]^CCGG[AT]
BsiEI 116 CGGCCG CG[GA][CT]^CG
BsmBI 3648 GAGACG CGTCTC(1/5)
Bsp1407I 875 TGTACA T^GTACA
BspHI 3644 TCATGA T^CATGA
BspLU11I 2759 ACATGT A^CATGT
BsrBI 2106 CCGCTC CCGCTC(-3/-3)
BsrFI 146 ACCGGC [GA]^CCGG[CT]
BsrGI 875 TGTACA T^GTACA
BssHII 4034 GCGCGC G^CGCGC
BstUI 4035 CGCG CG^CG
Bsu36I 3936 CCTCAGG CC^T[ACGT]AGG
CelII 150 GCTGAGC GC^T[ACGT]AGC
Cfr10I 146 ACCGGC [GA]^CCGG[CT]
DraI 3880 TTTAAA TTT^AAA
DraIII 3302 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 116 CGGCCG C^GGCCG
EclXI 116 CGGCCG C^GGCCG
Eco47III 3497 AGCGCT AGC^GCT
Eco52I 116 CGGCCG C^GGCCG
Eco81I 3936 CCTCAGG CC^T[ACGT]AGG
EcoRI 2472 GAATTC G^AATTC
FbaI 3716 TGATCA T^GATCA
HaeII 3497 AGCGCT [GA]GCGC^[CT]
HindIII 357 AAGCTT A^AGCTT
MfeI 1390 CAATTG C^AATTG
MluNI 3325 TGGCCA TGG^CCA
MscI 3325 TGGCCA TGG^CCA
MunI 1390 CAATTG C^AATTG
MvnI 4035 CGCG CG^CG
NcoI 730 CCATGG C^CATGG
NotI 115 GCGGCCGC GC^GGCCGC
PaeR7I 3434 CTCGAG C^TCGAG
PflFI 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2787 GAGTC GAGTC(4/5)
PshAI 68 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3644 TCATGA T^CATGA
ScaI 1700 AGTACT AGT^ACT
SpeI 2844 ACTAGT A^CTAGT
SspBI 875 TGTACA T^GTACA
StuI 3576 AGGCCT AGG^CCT
Tth111I 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 377 CCATCCTGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3434 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbsI 1735 GAAGAC GAAGAC(2/6)
737 GTCTTC
BglI 103 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
106 GCCGTGGCGGC
BpuAI 1735 GAAGAC GAAGAC(2/6)
737 GTCTTC
BsmI 1719 GAATGC GAATGC(1/-1)
4294 GAATGC
HincII 204 GTTGAC GT[CT]^[GA]AC
2899 GTTGAC
HindII 204 GTTGAC GT[CT]^[GA]AC
2899 GTTGAC
NdeI 464 CATATG CA^TATG
2243 CATATG
NheI 133 GCTAGC G^CTAGC
265 GCTAGC
SacI 1223 GAGCTC GAGCT^C
3920 GAGCTC
SmaI 96 CCCGGG CCC^GGG
138 CCCGGG
SspI 2383 AATATT AAT^ATT
4302 AATATT
XmaCI 96 CCCGGG C^CCGGG
138 CCCGGG
XmaI 96 CCCGGG C^CCGGG
138 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI