Restriction map for
pFN21AE3178
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII BalI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII Cfr13I CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
FauI FbaI FseI FspI HaeIII
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NciI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SfcI SfiI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 59 CGCG CG^CG
AcsI 664 GAATTC [GA]^AATT[CT]
AcyI 1130 GGCGCC G[GA]^CG[CT]C
AfaI 1052 GTAC GT^AC
AflIII 908 ACATGT A^C[GA][CT]GT
AocI 295 CCTGAGG CC^T[ACGT]AGG
ApoI 664 GAATTC [GA]^AATT[CT]
AseI 493 ATTAAT AT^TAAT
AsnI 493 ATTAAT AT^TAAT
AvrII 861 CCTAGG C^CTAGG
BamHI 300 GGATCC G^GATCC
BanII 291 GAGCCC G[GA]GC[CT]^C
BbeI 1130 GGCGCC GGCGC^C
BglI 955 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1020 AGATCT A^GATCT
BlnI 861 CCTAGG C^CTAGG
BsaHI 1130 GGCGCC G[GA]^CG[CT]C
BsaWI 11 TCCGGT [AT]^CCGG[AT]
BsmAI 1101 GAGAC GTCTC(1/5)
BsmFI 874 GTCCC GGGAC(10/14)
BspDI 559 ATCGAT AT^CGAT
BspLU11I 908 ACATGT A^CATGT
BsrBI 76 CCGCTC CCGCTC(-3/-3)
BsrFI 30 GCCGGT [GA]^CCGG[CT]
BsrI 1108 CCAGT ACTGG(1/-1)
BstAPI 27 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 881 TTCGAA TT^CGAA
BstUI 59 CGCG CG^CG
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
Cfr10I 30 GCCGGT [GA]^CCGG[CT]
ClaI 559 ATCGAT AT^CGAT
DdeI 296 CTGAG C^T[ACGT]AG
DsaI 1037 CCATGG C^C[GA][CT]GG
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
EcoRI 664 GAATTC G^AATTC
Hin1I 1130 GGCGCC G[GA]^CG[CT]C
HinfI 1090 GAGTC G^A[ACGT]TC
KasI 1130 GGCGCC G^GCGCC
MaeII 575 ACGT A^CGT
MslI 355 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 59 CGCG CG^CG
NarI 1130 GGCGCC GG^CGCC
NcoI 1037 CCATGG C^CATGG
NspV 881 TTCGAA TT^CGAA
PleI 1090 GAGTC GAGTC(4/5)
PshBI 493 ATTAAT AT^TAAT
RsaI 1052 GTAC GT^AC
SexAI 635 ACCTGGT A^CC[AT]GGT
SfoI 1130 GGCGCC GGC^GCC
SfuI 881 TTCGAA TT^CGAA
SphI 485 GCATGC GCATG^C
SspI 443 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanI 33 GGTGCC G^G[CT][GA]CC
1130 GGCGCC
BbsI 781 GAAGAC GAAGAC(2/6)
835 GAAGAC
BpmI 644 CTCCAG CTGGAG(16/14)
937 CTGGAG
BpuAI 781 GAAGAC GAAGAC(2/6)
835 GAAGAC
BstYI 300 GGATCC [GA]^GATC[CT]
1020 AGATCT
Cac8I 168 GCTGGC GC[ACGT]^[ACGT]GC
485 GCATGC
EcoT14I 861 CCTAGG C^C[AT][AT]GG
1037 CCATGG
EcoT22I 116 ATGCAT ATGCA^T
596 ATGCAT
HaeII 678 AGCGCC [GA]GCGC^[CT]
1130 GGCGCC
HphI 520 GGTGA GGTGA(8/7)
588 GGTGA
MflI 300 GGATCC [GA]^GATC[CT]
1020 AGATCT
NdeI 599 CATATG CA^TATG
727 CATATG
NsiI 116 ATGCAT ATGCA^T
596 ATGCAT
StyI 861 CCTAGG C^C[AT][AT]GG
1037 CCATGG
Tsp45I 521 GTGAC ^GT[GC]AC
857 GTGAC
XcmI 566 CCACCTGTAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1037 CCATGGAAGGAGTGG
XhoII 300 GGATCC [GA]^GATC[CT]
1020 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI CfoI DpnII
EcoRII Fnu4HI FokI HapII HhaI
HinP1I HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I